Q: Hello, does this product (NB300-251) only recognize the cleavage Notch1?

A: This antibody with recognize full length Notch1 if it is in the mix, as well as the cleaved product.

Human Notch-1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # KA2219

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Cell Lysate, Plasma, Serum (100 uL)

Sensitivity

n/a

Assay Range

79 to 5000 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

Quality control test: Standard curve. NOTCH1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human NOTCH1.

Scientific Data Images for Human Notch-1 ELISA Kit (Colorimetric)

ELISA: Human Notch-1 ELISA Kit (Colorimetric) [KA2219]

ELISA: Notch-1 ELISA Kit [Biotin] [KA2219] - Standard curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Notch-1

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane which then translocates to the nucleus to activate transcription of downstream genes.

Alternate Names

Notch1, TAN1

Gene Symbol

NOTCH1

Additional Notch-1 Products

Product Documents for Human Notch-1 ELISA Kit (Colorimetric)

Download Product Datasheets

Download Manual - Human Notch-1 ELISA Kit (Colorimetric)

Product Specific Notices for Human Notch-1 ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.