Notch-2 Antibody (B6) - Chimeric - Azide and BSA Free

Name: Notch-2 Antibody (B6) - Chimeric - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-62559
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-62559

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, ELISA, Block/Neutralize, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # B6

Format

Azide and BSA Free

View all Notch-2 Primary Antibodies »

Product Specifications

Immunogen

NRR of mouse Notch 2

Specificity

An engineered ScFv fragment antibody that binds to both mouse mNRR2 and human hNRR2, but not NRR1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Notch-2 Antibody (B6) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Notch-2 Antibody (B6) - Chimeric [NBP2-62559] -

Immunocytochemistry/Immunofluorescence: Notch-2 Antibody (B6) - Chimeric [NBP2-62559] - Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B6. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B6 (NBP2-62559) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62559, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

Applications for Notch-2 Antibody (B6) - Chimeric - Azide and BSA Free

Application

Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Application Notes

This chimeric rabbit antibody was made using the variable domain sequences of the original human scFv format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Notch-2

Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membraneFunctions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Alternate Names

Notch2

Gene Symbol

NOTCH2

Additional Notch-2 Products

Product Documents for Notch-2 Antibody (B6) - Chimeric - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for Notch-2 Antibody (B6) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.