Nox4 Antibody - BSA Free

A:

NB110-58849 is validated in the same species (apart from porcine) and applications as NB110-58851 and should be a suitable alternative for you. NB110-58851 was raised to a synthetic peptide made to a C-terminal region (within residues 500-578) of the human NOX4 protein, whilst NB110-58849 was raised to a synthetic peptide made to an internal region (between residues 100-200) of the human NOX4 protein. Both antibodies are rabbit polyclonals. As NB110-58849 has not yet been tested in porcine samples, you may be interested in our Innovator's Reward programme. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply need to go to the antibody's webpage and complete an online review with an image, detailing the positive or negative results of your study. In return you will receive a discount voucher for 100% of the purchase price of the reviewed product. More details of this programme can be found here: Innovator's Reward Program. I ran an alignment for you between the human NOX4 sequence and an unreviewed porcine NOX4 sequence from Uniprot (accession number F1STQ7.). The homology is high between the two proteins. Please do, however, be aware that this porcine sequence is unreviewed.

A:

I am delighted to hear you are pleased with our image! The fixation method used is Paraffin embedded with a dilution of 10-20 ug/ml. Please see our general protocol. This antibody has been tested by one of our innovators so we do not have their protocol. I would suggest fixing in 2% paraformaldehyde over night and citrate antigen retrieval as this is the most common form of antigen retrieval. If you face any problems through out your staining we are more than happy to help and can suggest alternative methods to gain a good level of staining.

A: Unfortunately the peptide sequence for this antibody is deemed proprietary and cannot be provided. However, we can tell you that it is a synthetic peptide made to a C-terminal region (within residues 500-578) of the human NOX4 protein sequence [Swiss-Prot# Q9NPH5]. Most likely this peptide will be approx. 10-20 amino acids long within that range.

A:

NB110-58849 is validated in the same species (apart from porcine) and applications as NB110-58851 and should be a suitable alternative for you. NB110-58851 was raised to a synthetic peptide made to a C-terminal region (within residues 500-578) of the human NOX4 protein, whilst NB110-58849 was raised to a synthetic peptide made to an internal region (between residues 100-200) of the human NOX4 protein. Both antibodies are rabbit polyclonals. As NB110-58849 has not yet been tested in porcine samples, you may be interested in our Innovator's Reward programme. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply need to go to the antibody's webpage and complete an online review with an image, detailing the positive or negative results of your study. In return you will receive a discount voucher for 100% of the purchase price of the reviewed product. More details of this programme can be found here: Innovator's Reward Program. I ran an alignment for you between the human NOX4 sequence and an unreviewed porcine NOX4 sequence from Uniprot (accession number F1STQ7.). The homology is high between the two proteins. Please do, however, be aware that this porcine sequence is unreviewed.

A:

I am delighted to hear you are pleased with our image! The fixation method used is Paraffin embedded with a dilution of 10-20 ug/ml. Please see our general protocol. This antibody has been tested by one of our innovators so we do not have their protocol. I would suggest fixing in 2% paraformaldehyde over night and citrate antigen retrieval as this is the most common form of antigen retrieval. If you face any problems through out your staining we are more than happy to help and can suggest alternative methods to gain a good level of staining.

A: Unfortunately the peptide sequence for this antibody is deemed proprietary and cannot be provided. However, we can tell you that it is a synthetic peptide made to a C-terminal region (within residues 500-578) of the human NOX4 protein sequence [Swiss-Prot# Q9NPH5]. Most likely this peptide will be approx. 10-20 amino acids long within that range.

A:

NB110-58849 is validated in the same species (apart from porcine) and applications as NB110-58851 and should be a suitable alternative for you. NB110-58851 was raised to a synthetic peptide made to a C-terminal region (within residues 500-578) of the human NOX4 protein, whilst NB110-58849 was raised to a synthetic peptide made to an internal region (between residues 100-200) of the human NOX4 protein. Both antibodies are rabbit polyclonals. As NB110-58849 has not yet been tested in porcine samples, you may be interested in our Innovator's Reward programme. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply need to go to the antibody's webpage and complete an online review with an image, detailing the positive or negative results of your study. In return you will receive a discount voucher for 100% of the purchase price of the reviewed product. More details of this programme can be found here: Innovator's Reward Program. I ran an alignment for you between the human NOX4 sequence and an unreviewed porcine NOX4 sequence from Uniprot (accession number F1STQ7.). The homology is high between the two proteins. Please do, however, be aware that this porcine sequence is unreviewed.

A:

I am delighted to hear you are pleased with our image! The fixation method used is Paraffin embedded with a dilution of 10-20 ug/ml. Please see our general protocol. This antibody has been tested by one of our innovators so we do not have their protocol. I would suggest fixing in 2% paraformaldehyde over night and citrate antigen retrieval as this is the most common form of antigen retrieval. If you face any problems through out your staining we are more than happy to help and can suggest alternative methods to gain a good level of staining.

A: Unfortunately the peptide sequence for this antibody is deemed proprietary and cannot be provided. However, we can tell you that it is a synthetic peptide made to a C-terminal region (within residues 500-578) of the human NOX4 protein sequence [Swiss-Prot# Q9NPH5]. Most likely this peptide will be approx. 10-20 amino acids long within that range.