Npas4 Antibody - Unpurified

Novus Biologicals | Catalog # NBP1-06574

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Mouse, Rat, Sheep

Cited:

Ovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Unpurified
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Product Specifications

Immunogen

Synthetic peptide derived from Cterm domain of mouse NPAS4 protein

Specificity

Reacts specifically with mouse 87 kDa protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Npas4 Antibody - Unpurified

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:5000

Reviewed Applications

Read 1 review rated 3 using NBP1-06574 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Lyophilized powder, reconstituted in distilled water.

Formulation

Antiserum

Format

Unpurified

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Npas4

Npas4 acts as a transcriptional activator in the presence of ARNT. It can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.

Alternate Names

BHLHE79, bHLHe79neuronal PAS domain-containing protein 4, Class E basic helix-loop-helix protein 79, Le-PAS, neuronal PAS domain protein 4, Neuronal PAS4, NXFHLH-PAS transcription factor NXF, PASD10PAS domain-containing protein 10

Entrez Gene IDs

225872 (Mouse)

Gene Symbol

NPAS4

UniProt

Additional Npas4 Products

Product Documents for Npas4 Antibody - Unpurified

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Npas4 Antibody - Unpurified

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Npas4 Antibody - Unpurified

Customer Reviews for Npas4 Antibody - Unpurified (1)

3 out of 5
1 Customer Rating
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Customer Images


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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Rat brain lysate (ventral hippocampus)
    Species: Rat
    Verified Customer | Posted 07/02/2013
    Npas4 Antibody - Unpurified NBP1-06574

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Npas4 Antibody - Unpurified

Showing  1 - 2 of 2 FAQs Showing All
  • Q: I would like to know the sequence of the synthetic peptide used to make the Npas4 antibody (cat. # NBP1-06574). It says on the product sheet that it was raised against the C-terminus, but I would like to know the specific sequence, if it is known and available.

    A: The exact sequence of NBP1-06574 is proprietary, but I can offer you the amino acid range. The immunogen falls between amino acids 600- 700 of Mouse Npas4 (accession number Q8BGD7).

  • Q: May we ask if any IHC image produced by NBP1-06574 can be provided for a reference?

    A: Unfortunately we do not have an IHC image for the Npas4 antibody, catalog number NBP1-06574, however we do stand behind the use of this antibody in IHC with a 100% guarantee.

  • Q: I would like to know the sequence of the synthetic peptide used to make the Npas4 antibody (cat. # NBP1-06574). It says on the product sheet that it was raised against the C-terminus, but I would like to know the specific sequence, if it is known and available.

    A: The exact sequence of NBP1-06574 is proprietary, but I can offer you the amino acid range. The immunogen falls between amino acids 600- 700 of Mouse Npas4 (accession number Q8BGD7).

  • Q: May we ask if any IHC image produced by NBP1-06574 can be provided for a reference?

    A: Unfortunately we do not have an IHC image for the Npas4 antibody, catalog number NBP1-06574, however we do stand behind the use of this antibody in IHC with a 100% guarantee.

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