Nuclear Pore Complex Protein Nsp1p Antibody (32D6) - BSA Free

Novus Biologicals | Catalog # NBP1-05227

Novus Biologicals
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Key Product Details

Species Reactivity

Yeast

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 32D6

Format

BSA Free
View all Nuclear Pore Complex Protein Nsp1p Primary Antibodies »

Product Specifications

Immunogen

Yeast nuclear preparations

Reactivity Notes

S. cerevisiae

Marker

Nuclear Envelope Marker, Nuclear Pore Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

86.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nuclear Pore Complex Protein Nsp1p Antibody (32D6) - BSA Free

Western Blot: Nuclear Pore Complex Protein Nsp1p Antibody (32D6) [NBP1-05227]

Western Blot: Nuclear Pore Complex Protein Nsp1p Antibody (32D6) [NBP1-05227]

Western Blot: Nuclear Pore Complex Protein Nsp1p Antibody [NBP1-05227] - Western Blot analysis of whole yeast protein extracts detects Nuclear Pore Complex Protein Nsp1p. Molecular weight detected is 86.5 kDa.

Applications for Nuclear Pore Complex Protein Nsp1p Antibody (32D6) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000-1:10000

Immunohistochemistry

1:1000-1:10000

Western Blot

1:10000-1:50000
Application Notes
This Nuclear Pore Complex Protein Nsp1p (32D6) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Supplied as concentrated hybridoma cell culture media.

Format

BSA Free

Preservative

5mM Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nuclear Pore Complex Protein Nsp1p

Nsp1p represents one of the most abundant nucleoporins (Nups) and is the only Nups member known to form two distinct NPC subcomplexes which are (i) Nic96p: this complex is composed of Nsp1p, Nup57p, Nup49p, and Nic96p, and is localized to both sides of the central gated channel and to the nuclear basket (ii) Nup82p: this complex is consists of Nsp1p, Nup82p, and Nup159p and is found exclusively on the cytoplasmic phase of nuclear pore complex. Moreover, Nup82p complex has also been documented to associate with Nup116p-Gle2p subcomplex. Both of these Nsp1p complexes (Nic96p and Nup82p) are implicated in delivering critical roles in nucleo-cytoplasmic transport. Genetic mutation or induced depletion of Nsp1p as well as of Nic96p, Nup57p, or Nup49p results in defective protein import. Nup82 or Nup159 mutants depict an expediated onset of nuclear mRNA retention with no impact on nuclear protein import. Moreover, Nsp1p, Nup49p and Nic96p appear to implicate in export of ribosomal large subunits, whereas Nup49 mutants are impaired in mRNA export.

Alternate Names

107 kDa nucleoporin, NPC proteins, Nuclear pore complex protein Nup107, Nucleoporin 107kDa, Nucleoporin Nup107, NUP 107, NUP 84, NUP107, NUP153, NUP155, NUP84, NUP98

Additional Nuclear Pore Complex Protein Nsp1p Products

Product Documents for Nuclear Pore Complex Protein Nsp1p Antibody (32D6) - BSA Free

Certificate of Analysis

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Product Specific Notices for Nuclear Pore Complex Protein Nsp1p Antibody (32D6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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