Nucleobindin 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-38066

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Nucleobindin 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 302-461 of human Nucleobindin 1 (NP_006175.2).

Sequence:
KNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWETVEMHPAYTEEELRRFEEELAAREAELNAKAQRLSQETEALGRSQGRLEAQKRELQQAVLHMEQRKQQQQQQQGHKAPAAHPEGQLKFHPDTDDVPVPAPAGDQKEVDTSEKKLLERLPEVEVPQHL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nucleobindin 1 Antibody - BSA Free

Nucleobindin 1 Antibody

Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP3-38066] -

Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP3-38066] - Immunofluorescence analysis of L929 cells using [KO Validated] Nucleobindin 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Nucleobindin 1 Antibody

Western Blot: Nucleobindin 1 Antibody [NBP3-38066] -

Western Blot: Nucleobindin 1 Antibody [NBP3-38066] - Western blot analysis of various lysates using [KO Validated] Nucleobindin 1 Rabbit pAb at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Nucleobindin 1 Antibody

Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP3-38066] -

Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP3-38066] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] Nucleobindin 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Nucleobindin 1 Antibody

Western Blot: Nucleobindin 1 Antibody [NBP3-38066] -

Western Blot: Nucleobindin 1 Antibody [NBP3-38066] - Western Blot analysis of lysates from wild type (WT) and Nucleobindin 1 knockout (KO) 293T cells, using [KO Validated] Nucleobindin 1 Rabbit pAb at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Applications for Nucleobindin 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nucleobindin 1

Nucleobindin 1 is a major calcium binding protein of the Golgi. It may have a role in calcium homeostasis.

Alternate Names

DKFZp686A15286, FLJ40471, NUCCALNUC, nucleobindin 1, nucleobindin-1

Gene Symbol

NUCB1

Additional Nucleobindin 1 Products

Product Documents for Nucleobindin 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Nucleobindin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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