Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all NUDC Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human NUDC (Accession#: NP_006591.1; Met1-Asn331)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for NUDC Antibody - Azide and BSA Free

Western Blot: NUDC Antibody [NBP2-96997]

Western Blot: NUDC Antibody [NBP2-96997]

Western Blot: NUDC Antibody [NBP2-96997] - Anti-NUDC rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: NIH3T3 Whole Cell Lysate. Lane D: Raw264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 46 kDa
Immunohistochemistry-Paraffin: NUDC Antibody [NBP2-96997]

Immunohistochemistry-Paraffin: NUDC Antibody [NBP2-96997]

Immunohistochemistry-Paraffin: NUDC Antibody [NBP2-96997] - Staining of human NUDC in human kidney with rabbit polyclonal antibody (1:5000).
Immunohistochemistry-Paraffin: NUDC Antibody [NBP2-96997]

Immunohistochemistry-Paraffin: NUDC Antibody [NBP2-96997]

Immunohistochemistry-Paraffin: NUDC Antibody [NBP2-96997] - Staining of human NUDC in human brain with rabbit polyclonal antibody (1:5000).
Immunoprecipitation: NUDC Antibody [NBP2-96997]

Immunoprecipitation: NUDC Antibody [NBP2-96997]

Immunoprecipitation: NUDC Antibody [NBP2-96997] - NUDC-His(C) was immunoprecipitated using: Lane A: 0.5 mg Raw264.7 Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. Lane C: 0.5 mg Hela Whole Cell Lysate4 ul anti-NUDC-His(C) rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NUDC-His(C) rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 42 kDa
NUDC Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: NUDC Antibody [NBP2-96997] -

Immunocytochemistry/Immunofluorescence: NUDC Antibody [NBP2-96997] - Immunofluorescence staining of NUDC in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-96997 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Applications for NUDC Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:2500-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NUDC

NudC was first identified as a regulator of nuclear movement in the asexual reproductive cycle of the filamentous fungus Aspergillus nidulans. Human NUDC is a nuclear movement protein that associates with dynein (see DYNC1H1; MIM 600112) (Aumais et al., 2003 [PubMed 12679384]).[supplied by OMIM]

Alternate Names

HNUDC, MNUDC, NPD011, nuclear distribution gene C (A.nidulans) homolog, nuclear distribution gene C homolog (A. nidulans), Nuclear distribution protein C homolog, nuclear migration protein nudC, NudC

Gene Symbol

NUDC

Additional NUDC Products

Product Documents for NUDC Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NUDC Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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