NUP133 Antibody (3E8) - Azide and BSA Free

Novus Biologicals | Catalog # H00055746-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E8

Format

Azide and BSA Free
View all NUP133 Primary Antibodies »

Product Specifications

Immunogen

NUP133 (NP_060700, 1069 a.a. ~ 1155 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LKLEILCKALQRDNWSSSDGKDDPIEVSKDSIFVKILQKLLKDGIQLSEYLPEVKDLLQADQLGSLKSNPYFEFVLKANYEYYVQGQ

Specificity

NUP133 (3E8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for NUP133 Antibody (3E8) - Azide and BSA Free

Western Blot: NUP133 Antibody (3E8) [H00055746-M01]

Western Blot: NUP133 Antibody (3E8) [H00055746-M01]

Western Blot: NUP133 Antibody (3E8) [H00055746-M01] - NUP133 monoclonal antibody (M01), clone 3E8 Analysis of NUP133 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: NUP133 Antibody (3E8) [H00055746-M01]

Immunocytochemistry/ Immunofluorescence: NUP133 Antibody (3E8) [H00055746-M01]

Immunocytochemistry/Immunofluorescence: NUP133 Antibody (3E8) [H00055746-M01] - Immunofluorescence of monoclonal antibody to NUP133 on HeLa cell. [antibody concentration 10 ug/ml]
Immunohistochemistry-Paraffin: NUP133 Antibody (3E8) [H00055746-M01]

Immunohistochemistry-Paraffin: NUP133 Antibody (3E8) [H00055746-M01]

Immunohistochemistry-Paraffin: NUP133 Antibody (3E8) [H00055746-M01] - Analysis of monoclonal antibody to NUP133 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
ELISA: NUP133 Antibody (3E8) [H00055746-M01]

ELISA: NUP133 Antibody (3E8) [H00055746-M01]

ELISA: NUP133 Antibody (3E8) [H00055746-M01] - Detection limit for recombinant GST tagged NUP133 is 0.03 ng/ml as a capture antibody.

Applications for NUP133 Antibody (3E8) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NUP133

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.

Alternate Names

FLJ10814, hNUP133,133 kDa nucleoporin, MGC21133, nuclear pore complex protein Nup133, nucleoporin 133kD, nucleoporin 133kDa, Nucleoporin Nup133

Entrez Gene IDs

55746 (Human)

Gene Symbol

NUP133

OMIM

607613 (Human)

UniProt

NP_060700 (Human)

Additional NUP133 Products

Product Documents for NUP133 Antibody (3E8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NUP133 Antibody (3E8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NUP133 Antibody (3E8) - Azide and BSA Free

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Protocols

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FAQs

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