Nup53 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-93322

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Nup53 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 276 and 376 of human nucleoporin 35kDa using the numbering given in entry NP_612142.2 (GeneID 129401).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nup53 Antibody - BSA Free

Western Blot: Nup53 Antibody [NB100-93322]

Western Blot: Nup53 Antibody [NB100-93322]

Western Blot: Nup53 Antibody [NB100-93322] - Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP35 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/ Immunofluorescence: Nup53 Antibody [NB100-93322]

Immunocytochemistry/ Immunofluorescence: Nup53 Antibody [NB100-93322]

Immunocytochemistry/Immunofluorescence: Nup53 Antibody [NB100-93322] - Showing nuclear co-localization with p300 in PANC-1 cells using anti-Nup53 antibody (green). Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Nup53 Antibody [NB100-93322]

Immunohistochemistry-Paraffin: Nup53 Antibody [NB100-93322]

Immunohistochemistry-Paraffin: Nup53 Antibody [NB100-93322] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- NUP35 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: Nup53 Antibody [NB100-93322]

Immunoprecipitation: Nup53 Antibody [NB100-93322]

Immunoprecipitation: Nup53 Antibody [NB100-93322] - Detection of human NUP35 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NUP35 antibody NB100-93322 (lot NB100-93322-2) used for IP at 6 ug per reaction. NUP35 was also immunoprecipitated by a previous lot of this antibody (lot NB100-93322-1) and another rabbit anti-NUP35 antibody. For blotting immunoprecipitated NUP35, NB100-93322 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Nup53 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250- 1:1000

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Nup53 antibody validated for ICC/IF from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB100-93322 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Nup53

This gene encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is part of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. [provided by RefSeq]

Alternate Names

35 kDa nucleoporin, Mitotic phosphoprotein 44, MP-44, MP44NUP53, NP44, Nuclear pore complex protein Nup53, nucleoporin 35kDa, Nucleoporin Nup35, nucleoporin NUP53

Entrez Gene IDs

129401 (Human)

Gene Symbol

NUP35

UniProt

NP_612142.2 (Human)

Additional Nup53 Products

Product Documents for Nup53 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Nup53 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nup53 Antibody - BSA Free

Customer Reviews for Nup53 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
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4 Stars
0%
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1 Stars
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Customer Images

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  • Name: Peibin Yue
    Application: Immunofluorescence
    Sample Tested: Human Panc-1 Cell Line
    Species: Human
    Verified Customer | Posted 11/24/2014
    Immunofluorescence: Nup35 Antibody[NB100-93322](Green) showing nuclear colocalization with p300 in Panc-1 cells.
    Nup53 Antibody - BSA Free NB100-93322

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