Occludin Antibody (CL1555) - BSA Free

Name: Occludin Antibody (CL1555) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-52971
Price: 329 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-52971

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # CL1555

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q16625, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Occludin Antibody (CL1555) - BSA Free

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Analysis in human stomach and skeletal muscle tissues using NBP2-52971 antibody. Corresponding OCLN RNA-seq data are presented for the same tissues.

Western Blot: Occludin Antibody (CL1555) [NBP2-52971]

Western Blot: Occludin Antibody (CL1555) [NBP2-52971] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: OCLN Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Immunocytochemistry/ Immunofluorescence: Occludin Antibody (CL1555) [NBP2-52971]

Immunocytochemistry/Immunofluorescence: Occludin Antibody (CL1555) [NBP2-52971] - staining of CACO-2 cells using the Anti-OCLN monoclonal antibody, showing specific staining in cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human prostate cancer shows strong apical membrane positivity in tumor cells.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human kidney shows membranous positivity in renal tubules.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human liver shows membranous immunoreactivity in hepatocytes.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human placenta shows membranous immunoreactivity in the trophoblast.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human stomach shows strong positivity in apical membrane in glandular cells.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human colon shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human fallopian tube shows strong positivity in apical membrane in glandular cells.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human kidney shows moderate to strong positivity in apical membrane in cells in tubules.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

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Applications for Occludin Antibody (CL1555) - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Occludin

The Occludin gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.

Alternate Names

BLCPMG, OCLN

Gene Symbol

OCLN

Additional Occludin Products

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Product Specific Notices for Occludin Antibody (CL1555) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.