Olig2 Antibody (1G11)

Novus Biologicals | Catalog # NBP2-37636

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1G11
View all Olig2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Olig2 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Olig2 Antibody (1G11)

Western Blot: Olig2 Antibody (1G11)BSA Free [NBP2-37636]

Western Blot: Olig2 Antibody (1G11)BSA Free [NBP2-37636]

Western Blot: Olig2 Antibody (1G11) [NBP2-37636] - Western blot analysis using OLIG2 mAb against HEK293 (1) and OLIG2(AA: 1-122)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Olig2 Antibody (1G11) - BSA Free [NBP2-37636]

Immunocytochemistry/ Immunofluorescence: Olig2 Antibody (1G11) - BSA Free [NBP2-37636]

Immunocytochemistry/Immunofluorescence: Olig2 Antibody (1G11) [NBP2-37636] - Immunofluorescence analysis of U251 cells using OLIG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: Olig2 Antibody (1G11) - BSA Free [NBP2-37636]

Immunohistochemistry: Olig2 Antibody (1G11) - BSA Free [NBP2-37636]

Immunohistochemistry: Olig2 Antibody (1G11) [NBP2-37636] - Immunohistochemical analysis of paraffin-embedded muscle tissues using OLIG2 mouse mAb with DAB staining.
ELISA: Olig2 Antibody (1G11) - BSA Free [NBP2-37636]

ELISA: Olig2 Antibody (1G11) - BSA Free [NBP2-37636]

ELISA: Olig2 Antibody (1G11) [NBP2-37636] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Olig2 Antibody (1G11)

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Olig2

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. OIlig2 is expressed in the ventral spinal cord as early as 9.5 dpc and is scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. (Arnett et al., 2004) From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).

Long Name

Oligodendrocyte Lineage Transcription Factor 2

Alternate Names

BHLHB1, PRKCBP2, RACK17

Gene Symbol

OLIG2

Additional Olig2 Products

Product Documents for Olig2 Antibody (1G11)

Certificate of Analysis

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Product Specific Notices for Olig2 Antibody (1G11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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