Olig2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-28667

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Olig2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant mouse Olig2. Accession # Q9EQW6

Marker

Oligodendrocyte Marker

Specificity

Specific for endogenous levels of the ~32 kDa Olig2 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for Olig2 Antibody - Azide and BSA Free

Immunohistochemistry: Olig2 Antibody - Azide and BSA Free [NBP1-28667]

Immunohistochemistry: Olig2 Antibody - Azide and BSA Free [NBP1-28667]

Immunohistochemistry: Olig2 Antibody [NBP1-28667] - Mouse cerebral microslice section (coronal, P14) was co-immunostained with anti-Olig2 and anti-NeuN antibodies. Green: Olig2, Red: NeuN. Anti-Olig2 was used at a 1:800 dilution. This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: Olig2 Antibody - Azide and BSA Free [NBP1-28667]

Immunocytochemistry/ Immunofluorescence: Olig2 Antibody - Azide and BSA Free [NBP1-28667]

Immunocytochemistry/Immunofluorescence: Olig2 Antibody [NBP1-28667] - Rat cortical neuroepithelial cells with anti-Olig2 (red). The cells were treated with basic FGF for 30 hours prior to staining (this induces Olig2 expression in these primary cells).
Immunohistochemistry-Frozen: Olig2 Antibody - Azide and BSA Free [NBP1-28667]

Immunohistochemistry-Frozen: Olig2 Antibody - Azide and BSA Free [NBP1-28667]

Immunohistochemistry-Frozen: Olig2 Antibody [NBP1-28667] - Mouse brain cortex. Antibody at 1:300. Purple: Olig2. Green: CD31. Image submitted by a verified customer review.
Olig2 Antibody - Azide and BSA Free

Western Blot: Olig2 Antibody - Azide and BSA Free [NBP1-28667] -

Western analysis of cell lysates.a Western analysis of primary neurons, astrocytes, microglia, and OPCs derived from WT and PINK1 KO rats. Cultured cells were treated with 100 nM valinomycin or DMSO control for 4 h at 37 C, then harvested for western analysis. Ten micrograms of cell lysate protein was loaded in each lane, resolved by SDS-PAGE, transferred to PVDF membrane, and blotted using anti-pS65-Ub antibody. The same membrane was re-probed using the antibodies indicated on the lower panels as markers for neurons ( beta 3-tubulin), astrocytes (ALDH1L1), microglia (Iba-1), and OPCs (Olig-2). b Mean +/− SEM pS65-Ub level measured by densitometry of western blot membranes from four independent experiments. (Four asterisks (****) indicate significantly different from all other groups by ANOVA followed by Tukey’s multiple comparisons test p < 0.001; hash (#) indicates significantly different from all other groups except valinomycin-treated WT and KO neurons and microglia, as well as DMSO-treated WT astrocytes p < 0.05). c Mean +/− SEM total ubiquitin levels from the same four membranes. d Mean +/− SEM pS65-Ub level normalized to the total ubiquitin level in each lane (Four asterisks (****) indicate significantly different from all other groups p < 0.0001; asterisk (*) indicates p < 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31840043), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Olig2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:800

Immunohistochemistry-Paraffin

1:1000

Immunoprecipitation

1:500-1:1000

Western Blot

1:3000
Application Notes
Use in IHC-FrFl reported in scientific literature (PMID:35476595) Olig2 antibody validated for IHC-FR from a verified customer review..

Reviewed Applications

Read 4 reviews rated 4.8 using NBP1-28667 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

200 mM Tris (pH 8.0), 0.1 M Glycine

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Olig2

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Expressed in the ventral spinal cord as early as 9.5 dpc and scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. (Arnett et al., 2004) From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).

Long Name

Oligodendrocyte Lineage Transcription Factor 2

Alternate Names

BHLHB1, PRKCBP2, RACK17

Entrez Gene IDs

10215 (Human); 50913 (Mouse)

Gene Symbol

OLIG2

UniProt

Q9EQW6 (Mouse)

Additional Olig2 Products

Product Documents for Olig2 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Olig2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Olig2 Antibody - Azide and BSA Free

Customer Reviews for Olig2 Antibody - Azide and BSA Free (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Olig2 Antibody
    Name: Abhijit Nirwane
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 04/17/2019
    Mouse brain cortex, Dilution factor 1:300, Purple, Olig2; Green, CD31
    Mice brain slices
    Olig2 Antibody - Azide and BSA Free NBP1-28667
  • Olig2 Antibody
    Name: Kazuya Oikawa
    Application: Immunohistochemistry-Frozen
    Sample Tested: Optic nerve
    Species: Feline
    Verified Customer | Posted 02/06/2019
    NBP1-28667 antibody showed nuclear staining as expected in oligodendrocytes (red). DAPI (blue). Image was captured with epi-fluorescent microscopy
    Olig2 Antibody - Azide and BSA Free NBP1-28667
  • Olig2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-microslice section
    Sample Tested: Brain (cerebral cortex)
    Species: Mouse
    Verified Customer | Posted 08/07/2017
    Mouse cerebral microslice section (coronal, P14) was co-immunostained with anti-Olig2 (NBP1-28667) and anti-NeuN antibodies. Green: Olig2, Red: NeuN.
    Anti-Olig2 was used at a 1/800 dilution.
    Olig2 Antibody - Azide and BSA Free NBP1-28667
  • Name: Nancy Harris
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Rat brain tumors
    Species: Rat
    Verified Customer | Posted 02/26/2013

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Protocols

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