Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chicken (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Olig2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid peptide near the amino terminus of human OLIG2. The immunogen is located within amino acids 80 - 130 of OLIG2. Amino Acid Squence: STKKDKKQMTEPELQ

Specificity

It is predicted to not cross-react with other members of the OLIG family of proteins. At least three isoforms of OLIG2 are known to exist; this antibody will detect all three isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Olig2 Antibody - BSA Free (NBP2-41269) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Olig2 Antibody - BSA Free

Western Blot: Olig2 AntibodyBSA Free [NBP2-41269]

Western Blot: Olig2 AntibodyBSA Free [NBP2-41269]

Western Blot: Olig2 Antibody [NBP2-41269] - Western blot analysis of OLIG2 in EL4 cell lysate with OLIG2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry: Olig2 Antibody - BSA Free [NBP2-41269]

Immunohistochemistry: Olig2 Antibody - BSA Free [NBP2-41269]

Immunohistochemistry: Olig2 Antibody [NBP2-41269] - Staining of OLIG2 in mouse brain tissue with OLIG2 Antibodyat 5 ug/mL.
Olig2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Olig2 Antibody - BSA Free [NBP2-41269] -

Immunocytochemistry/ Immunofluorescence: Olig2 Antibody - BSA Free [NBP2-41269] - Immunofluorescence of Olig2 in mouse brain tissue with Olig2 Antibody at 20 ug/mL.

Green: Olig2 antibody
Red: Phylloidin staining
Blue: DAPI staining
Olig2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Olig2 Antibody - BSA Free [NBP2-41269] -

Immunocytochemistry/ Immunofluorescence: Olig2 Antibody - BSA Free [NBP2-41269] - Immunofluorescence of Olig2 in rat brain tissue with Olig2 antibody at 20 ug/mL.
Olig2 Antibody - BSA Free

Immunohistochemistry: Olig2 Antibody - BSA Free [NBP2-41269] -

Immunohistochemistry: Olig2 Antibody - BSA Free [NBP2-41269] - Immunohistochemistry of Olig2 in rat brain tissue with Olig2 antibody at 5 ug/mL.

Applications for Olig2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1 - 2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Olig2

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. OIlig2 is expressed in the ventral spinal cord as early as 9.5 dpc and is scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. (Arnett et al., 2004) From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).

Long Name

Oligodendrocyte Lineage Transcription Factor 2

Alternate Names

BHLHB1, PRKCBP2, RACK17

Gene Symbol

OLIG2

Additional Olig2 Products

Product Documents for Olig2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Olig2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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