Opsin 1 (Medium Wave) Antibody

Novus Biologicals | Catalog # NB110-74730

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Opsin 1 (Medium Wave) Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from mouse Opsin 1 (Medium Wave) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33803057).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Opsin 1 (Medium Wave) Antibody (NB110-74730) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Opsin 1 (Medium Wave) Antibody: Cited in 16 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Opsin 1 (Medium Wave) Antibody

Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730]

Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730]

Opsin-1-Medium-Wave-Antibody-Immunohistochemistry-NB110-74730-img0011.jpg
Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730]

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730]

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IF analysis of Opsin 1 in paraffin embedded and frozen mouse retina tissues. Image courtesy of product review submitted by Linda Vuong.
Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730]

Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730]

Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730] - Retinal whole mount of BALB/c mouse stained against anti M-opsin secondary (AF647) antibody and peanut agglutinin (Fluorescein). This image was submitted via customer Review.
Opsin 1 (Medium Wave) Antibody

Opsin 1 (Medium Wave) Antibody [NB110-74730] -

HC-P on paraffin sections of mouse eye. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Opsin 1 (Medium Wave) Antibody

Opsin 1 (Medium Wave) Antibody [NB110-74730] -

IHC-P on paraffin sections of mouse eye. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Applications for Opsin 1 (Medium Wave) Antibody

Application
Recommended Usage

Immunohistochemistry

1:250-1:500

Immunohistochemistry-Frozen

1:250-1:500

Immunohistochemistry-Paraffin

1:250-1:500

Western Blot

1:250-1:500
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30307502). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29162627).

Reviewed Applications

Read 3 reviews rated 4.7 using NB110-74730 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

0.02% Benzalkonium chloride

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Opsin 1 (Medium Wave)

FUNCTION: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. May increase spectral sensitivity in dim light. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in cone photoreceptor cells.

Alternate Names

GCP, GOP, Green cone photoreceptor pigment, Green-sensitive opsin, medium-wave-sensitive opsin 1, OPN1MW, opsin 1 (cone pigments), medium-wave-sensitive 2

Entrez Gene IDs

14539 (Mouse); 89810 (Rat)

Gene Symbol

OPN1MW

UniProt

O35599 (Mouse)

Additional Opsin 1 (Medium Wave) Products

Product Documents for Opsin 1 (Medium Wave) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Opsin 1 (Medium Wave) Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Opsin 1 (Medium Wave) Antibody

Customer Reviews for Opsin 1 (Medium Wave) Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Opsin 1 (Medium Wave) Antibody
    Name: Henri Leinonen
    Application: Immunohistochemistry of intact specimen
    Sample Tested: mouse retina
    Species: Mouse
    Verified Customer | Posted 07/18/2017
    Retinal whole mount of BALB/c mouse stained against anti M-opsin (Novus Biologicals NB110-74730; + AlexaFluor 647 nm secondary AB) antibody and peanut agglutinin (+ Fluorescein).
    Anti-rabbit AlexaFluor 647 nm secondary antibody
    Opsin 1 (Medium Wave) Antibody NB110-74730
  • Opsin 1 (Medium Wave) Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse retina embedded in paraffin
    Species: Mouse
    Verified Customer | Posted 06/21/2016
  • Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Mouse retina embedded in paraffin and frozen medium
    Species: Mouse
    Verified Customer | Posted 08/14/2012
    Opsin 1 (Medium Wave) Antibody NB110-74730

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Protocols

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FAQs for Opsin 1 (Medium Wave) Antibody

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  • Q: I would to know if the synthetic peptide used as immunogen to generate the anti-opsin1 antibody (NB110-74730) belongs to the N-terminus or C-terminus of opsin1?

    A: The antigen for our Opsin 1 (Medium Wave) antibody [NB110-74730] is located between aa region  2-50, so it is N-terminal.

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