Osteocalcin Antibody (2D4) - Azide and BSA Free

Novus Biologicals | Catalog # H00000632-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Lambda Clone # 2D4

Format

Azide and BSA Free
View all Osteocalcin Primary Antibodies »

Product Specifications

Immunogen

BGLAP (NP_954642, 52 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YLYQWLGAPVPYPDPLEPRREVCELNPDCDELADHIGFQEAYRRFYGPV

Specificity

BGLAP - bone gamma-carboxyglutamate (gla) protein (osteocalcin)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Lambda

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Osteocalcin Antibody (2D4) - Azide and BSA Free

Western Blot: Osteocalcin Antibody (2D4) [H00000632-M01]

Western Blot: Osteocalcin Antibody (2D4) [H00000632-M01]

Western Blot: Osteocalcin Antibody (2D4) [H00000632-M01] - Analysis of BGLAP expression in transfected 293T cell line by BGLAP monoclonal antibody (M01), clone 2D4.Lane 1: BGLAP transfected lysate(11 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Osteocalcin Antibody (2D4) [H00000632-M01]

Immunocytochemistry/ Immunofluorescence: Osteocalcin Antibody (2D4) [H00000632-M01]

Immunocytochemistry/Immunofluorescence: Osteocalcin Antibody (2D4) [H00000632-M01] - Analysis of monoclonal antibody to BGLAP on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: Osteocalcin Antibody (2D4) [H00000632-M01]

ELISA: Osteocalcin Antibody (2D4) [H00000632-M01]

ELISA: Osteocalcin Antibody (2D4) [H00000632-M01] - Detection limit for recombinant GST tagged BGLAP is approximately 0.3ng/ml as a capture antibody.
Osteocalcin Antibody (2D4)

Immunohistochemistry: Osteocalcin Antibody (2D4) [H00000632-M01] -

h00000632-m01_mouse-monoclonal-osteocalcin-antibody-2d4-25520231539515.jpg

Applications for Osteocalcin Antibody (2D4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
Use in IHC reported in scientific literature (PMID:34948393).Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been use for IF..

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Osteocalcin

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium

Long Name

Bone gamma-Carboxyglutamate [gla] Protein

Alternate Names

BGLAP, BGP, OCN

Entrez Gene IDs

632 (Human)

Gene Symbol

BGLAP

OMIM

112260 (Human)

UniProt

NP_954642 (Human)

Additional Osteocalcin Products

Product Documents for Osteocalcin Antibody (2D4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Osteocalcin Antibody (2D4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Osteocalcin Antibody (2D4) - Azide and BSA Free

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Protocols

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FAQs

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