Osteopontin/OPN Antibody (1B20) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80895

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Rabbit

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B20

Format

Azide and BSA Free
View all Osteopontin/OPN Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451].

Localization

Secreted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Osteopontin/OPN Antibody (1B20) - Azide and BSA Free

Western Blot: Osteopontin/OPN Antibody (1B20)Azide and BSA Free [NBP2-80895]

Western Blot: Osteopontin/OPN Antibody (1B20)Azide and BSA Free [NBP2-80895]

Western Blot: Osteopontin/OPN Antibody (1B20) - Azide and BSA Free [NBP2-80895] - Analysis of Osteopontin expression in U2OS whole cell lysate. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Osteopontin/OPN Antibody (1B20) - Azide and BSA Free [NBP2-80895]

Immunocytochemistry/ Immunofluorescence: Osteopontin/OPN Antibody (1B20) - Azide and BSA Free [NBP2-80895]

Immunocytochemistry/Immunofluorescence: Osteopontin/OPN Antibody (1B20) - Azide and BSA Free [NBP2-80895] - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red). Image from the standard format of this antibody.
Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - Azide and BSA Free [NBP2-80895]

Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - Azide and BSA Free [NBP2-80895]

Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - Azide and BSA Free [NBP2-80895] - Analysis of Osteopontin on human lung adenocarcinoma. Image from the standard format of this antibody.
Simple Western: Osteopontin/OPN Antibody (1B20)Azide and BSA Free [NBP2-80895]

Simple Western: Osteopontin/OPN Antibody (1B20)Azide and BSA Free [NBP2-80895]

Simple Western: Osteopontin/OPN Antibody (1B20) - Azide and BSA Free [NBP2-80895] - Simple Western lane view shows a specific band for Osteopontin in 0.5 mg/ml of Human Breast Cancer lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody Image from the standard format of this antibody.

Applications for Osteopontin/OPN Antibody (1B20) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

reported in scientific literature (PMID 24269728)

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10-1:500

Simple Western

1:20

Western Blot

1:1000
Application Notes
In Western blot multiple bands can be seen due to glycosylation and phosphorylation of the protein.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:20. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Osteopontin/OPN

The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood, and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been suggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody (1B20) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Osteopontin/OPN Antibody (1B20) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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