Osteopontin/OPN Antibody

Novus Biologicals | Catalog # NB100-1883

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Osteopontin/OPN Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DPKSKEEDKHLK corresponding to C-Terminus according to NP_001035147.1, NP_000573.1, NP_001035149.1.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:35063020). Mouse reactivity reported in scientific literature (PMID:23884286).

Localization

Secreted

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Osteopontin/OPN Antibody

Western Blot: Osteopontin/OPN Antibody [NB100-1883]

Western Blot: Osteopontin/OPN Antibody [NB100-1883]

Western Blot: Osteopontin/OPN Antibody [NB100-1883] - Staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Osteopontin/OPN Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1-0.3 ug/ml
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:35063020) WB: Approx 60kDa band observed in lysates of Human Tonsil and lysates of cell line MOLT4 and Jurkat (calculated MW of 33.8kDa according to NP_000573.1). The observed molecular weight corresponds to earlier findings in literature (Singh et al, J Biol Chem. 1995 Nov 24;270(47):28471-8; PMID: 7499354). Primary incubation was 1 hour. ICC/IF, IHC usage is reported in scientific literature (PMID: 23884286).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Entrez Gene IDs

6696 (Human); 20750 (Mouse)

Gene Symbol

SPP1

UniProt

p10451 (Human)

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody

Certificate of Analysis

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Product Specific Notices for Osteopontin/OPN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Osteopontin/OPN Antibody

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Protocols

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