Osteopontin/OPN Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-59190
Key Product Details
Species Reactivity
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Antibody Source
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Product Specifications
Immunogen
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Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Scientific Data Images for Osteopontin/OPN Antibody - BSA Free
Western Blot: Osteopontin/OPN Antibody [NBP1-59190]
Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Jurkat cell lysate, Antibody Titration: 1.25ug/mlImmunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-59190]
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-59190] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.Western Blot: Osteopontin/OPN Antibody [NBP1-59190]
Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5ug/mL, Peptide Concentration: 2.0ug/mL, Lysate Quantity: 25ug/lane, Gel Concentration: 12%Western Blot: Osteopontin/OPN Antibody - BSA Free [NBP1-59190] -
Western blot analysis of principal marker of bone homing. (A) Western blot analysis of Versican, Osteopontin, and Prolactin receptor in MCF-7 cells untreated and treated with IL-1 beta and AZA after 48 h. Representative images of WB and densitometric analysis (Mean +/- SD) of Versican, Osteopontin, and Prolactin receptor. One-way ANOVA showed significant effect of treatment on densitometric data (p < 0.0005); significant differences were highlighted among tested treatments (p < 0.0005), except for Prolactin receptor with AZA (NS). (B) Western blot analysis of Versican, Osteopontin, and Prolactin receptor in MCF-7 cells untreated and treated with IL-1 beta and Bobcat339 (33 uM and 75 uM) and relative co-treatments after 48 h. Representative images of WB and densitometric analysis (Mean +/- SD, n = 3) of Versican, Osteopontin, and Prolactin receptor. One-way ANOVA showed significant effect of treatment on densitometric data; significant differences among tested treatments versus untreated MCF-7 (*), and among IL-1 beta /BC co-treatments versus the IL-1 beta one () are reported in the graphs (*** and, p < 0.0005). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36499741), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: Osteopontin/OPN Antibody - BSA Free [NBP1-59190] -
Western blot analysis of principal marker of bone homing. (A) Western blot analysis of Versican, Osteopontin, and Prolactin receptor in MCF-7 cells untreated and treated with IL-1 beta and AZA after 48 h. Representative images of WB and densitometric analysis (Mean +/- SD) of Versican, Osteopontin, and Prolactin receptor. One-way ANOVA showed significant effect of treatment on densitometric data (p < 0.0005); significant differences were highlighted among tested treatments (p < 0.0005), except for Prolactin receptor with AZA (NS). (B) Western blot analysis of Versican, Osteopontin, and Prolactin receptor in MCF-7 cells untreated and treated with IL-1 beta and Bobcat339 (33 uM and 75 uM) and relative co-treatments after 48 h. Representative images of WB and densitometric analysis (Mean +/- SD, n = 3) of Versican, Osteopontin, and Prolactin receptor. One-way ANOVA showed significant effect of treatment on densitometric data; significant differences among tested treatments versus untreated MCF-7 (*), and among IL-1 beta /BC co-treatments versus the IL-1 beta one () are reported in the graphs (*** and, p < 0.0005). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36499741), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Osteopontin/OPN Antibody - BSA Free
Immunohistochemistry
Immunohistochemistry-Paraffin
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Format
Preservative
Concentration
Shipping
Stability & Storage
Background: Osteopontin/OPN
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional Osteopontin/OPN Products
Product Documents for Osteopontin/OPN Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for Osteopontin/OPN Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Osteopontin/OPN Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Osteopontin/OPN Antibody - BSA Free
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Q: I ordered this antibody and used it to detect osteopontin isolated from bovine milk. Three bands were detected when this antibody was used and one intense band showed similar molecular weight with casein. Since casein is abundant in milk, I am wondering if this osteopontin antibody has been tested to cross react with other proteins?
A: The immunogen for this antibody shows no similarity with casein, so is not predicted to cross-react. It hasn't been directly tested for this, but no other proteins show up in a BLAST of the immunogen, so most likely this is something that is uncharacterized.