Osteopontin/OPN Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-89952
![Immunocytochemistry/ Immunofluorescence: Osteopontin/OPN Antibody [NBP1-89952]](https://beta-resources.rndsystems.com/images/products/Osteopontin-OPN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89952-img0012.jpg "Immunocytochemistry/ Immunofluorescence: Osteopontin/OPN Antibody [NBP1-89952]")
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDAT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Osteopontin/OPN Antibody - BSA Free (NBP1-89952) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Osteopontin/OPN Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Osteopontin/OPN Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: Osteopontin/OPN Antibody [NBP1-89952]
Immunocytochemistry/Immunofluorescence: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human cell line Hep G2 shows localization to the Golgi apparatus. Antibody staining is shown in green.![Osteopontin/OPN Antibody - BSA Free Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody - BSA Free [NBP1-89952]](https://beta-resources.rndsystems.com/images/products/nbp1-89952_rabbit-polyclonal-osteopontin-opn-antibody-135202517442149.jpg "Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody - BSA Free [NBP1-89952]")
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952]](https://beta-resources.rndsystems.com/images/products/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-89952-img0007.jpg "Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952]")
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952]
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human placenta shows high expression.![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952]](https://beta-resources.rndsystems.com/images/products/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-89952-img0016.jpg "Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952]")
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952]
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human liver shows low positivity in hepatocytes as expected.![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952]](https://beta-resources.rndsystems.com/images/products/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-89952-img0017.jpg "Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952]")
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952]
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human tonsil shows low positivity in non-germinal center cells as expected.![Osteopontin/OPN Antibody - BSA Free Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody - BSA Free [NBP1-89952]](https://beta-resources.rndsystems.com/images/products/nbp1-89952_rabbit-polyclonal-osteopontin-opn-antibody-15520258273077.jpg "Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody - BSA Free [NBP1-89952]")
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody - BSA Free [NBP1-89952]
Staining of human kidney shows strong positivity in cytoplasm and secretion in tubules.![Osteopontin/OPN Antibody - BSA Free Western Blot: Osteopontin/OPN Antibody - BSA Free [NBP1-89952]](https://beta-resources.rndsystems.com/images/products/nbp1-89952_rabbit-polyclonal-osteopontin-opn-antibody-15520258251110.jpg "Western Blot: Osteopontin/OPN Antibody - BSA Free [NBP1-89952]")
Western Blot: Osteopontin/OPN Antibody - BSA Free [NBP1-89952]
Analysis in human cell line EFO-21.Applications for Osteopontin/OPN Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Osteopontin/OPN
Long Name
Secreted Phosphoprotein 1 [BNSP]
Alternate Names
Eta-1, OPN, Spp1
Gene Symbol
SPP1
Additional Osteopontin/OPN Products
Product Documents for Osteopontin/OPN Antibody - BSA Free
Product Specific Notices for Osteopontin/OPN Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Osteopontin/OPN Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
