Otx2 Antibody (1H12C4B5)

Novus Biologicals | Catalog # NBP2-37597

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H12C4B5
View all Otx2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Otx2 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Otx2 Antibody (1H12C4B5)

Western Blot: Otx2 Antibody (1H12C4B5)BSA Free [NBP2-37597]

Western Blot: Otx2 Antibody (1H12C4B5)BSA Free [NBP2-37597]

Western Blot: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Western blot analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: Otx2 Antibody (1H12C4B5) - BSA Free [NBP2-37597]

Immunocytochemistry/ Immunofluorescence: Otx2 Antibody (1H12C4B5) - BSA Free [NBP2-37597]

Immunocytochemistry/Immunofluorescence: Otx2 Antibody (1H12C4B5) [NBP2-37597] - ARPE-19 monolayer on glass substrate. Fixative 4% PFA Blocking Goat Serum Dilution 1:200. Incubation 1h @RT. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Otx2 Antibody (1H12C4B5) - BSA Free [NBP2-37597]

Immunohistochemistry-Paraffin: Otx2 Antibody (1H12C4B5) - BSA Free [NBP2-37597]

Immunohistochemistry-Paraffin: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Analysis of paraffin-embedded prostate tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.
Flow Cytometry: Otx2 Antibody (1H12C4B5) - BSA Free [NBP2-37597]

Flow Cytometry: Otx2 Antibody (1H12C4B5) - BSA Free [NBP2-37597]

Flow Cytometry: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Flow cytometric analysis of HepG2 cells using OTX2 mouse mAb (green) and negative control (purple).
Western Blot: Otx2 Antibody (1H12C4B5)BSA Free [NBP2-37597]

Western Blot: Otx2 Antibody (1H12C4B5)BSA Free [NBP2-37597]

Western Blot: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Western blot analysis using OTX2 mAb against human OTX2 (AA: 40-297) recombinant protein. (Expected MW is 65 kDa)
Immunocytochemistry/ Immunofluorescence: Otx2 Antibody (1H12C4B5) - BSA Free [NBP2-37597]

Immunocytochemistry/ Immunofluorescence: Otx2 Antibody (1H12C4B5) - BSA Free [NBP2-37597]

Immunocytochemistry/Immunofluorescence: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Immunofluorescence analysis of HepG2 cells using OTX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
ELISA: Otx2 Antibody (1H12C4B5) - BSA Free [NBP2-37597]

ELISA: Otx2 Antibody (1H12C4B5) - BSA Free [NBP2-37597]

ELISA: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Otx2 Antibody (1H12C4B5)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Reviewed Applications

Read 1 review rated 5 using NBP2-37597 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Otx2

Homeobox protein Otx2 is a member of the bicoid sub-family of homeodomain-containing transcription factors. It is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sense organs. Two distinct isoforms have been identified. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Long Name

Orthodenticle Homolog 2

Alternate Names

homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2

Gene Symbol

OTX2

Additional Otx2 Products

Product Documents for Otx2 Antibody (1H12C4B5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Otx2 Antibody (1H12C4B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Otx2 Antibody (1H12C4B5)

Customer Reviews for Otx2 Antibody (1H12C4B5) (1)

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Customer Images

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  • Otx2 Antibody (1H12C4B5)
    Name: Fabio Marongiu
    Application: Immunocytochemistry
    Sample Tested: ARPE-19 cells
    Species: Human
    Verified Customer | Posted 08/29/2017
    ARPE-19 monolayer on glass substrate
    Fixative 4% PFA Blocking Goat Serum Dilution 1:200 Incubation 1h @RT
    Otx2 Antibody (1H12C4B5) NBP2-37597

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Protocols

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FAQs

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