Otx2 Antibody (1H12G8B2)

Novus Biologicals | Catalog # NBP2-37596

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H12G8B2
View all Otx2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Otx2 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Otx2 Antibody (1H12G8B2)

Western Blot: Otx2 Antibody (1H12G8B2)BSA Free [NBP2-37596]

Western Blot: Otx2 Antibody (1H12G8B2)BSA Free [NBP2-37596]

Western Blot: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: Otx2 Antibody (1H12G8B2) - BSA Free [NBP2-37596]

Immunocytochemistry/ Immunofluorescence: Otx2 Antibody (1H12G8B2) - BSA Free [NBP2-37596]

Immunocytochemistry/Immunofluorescence: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis of U251 cells using OTX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Otx2 Antibody (1H12G8B2) - BSA Free [NBP2-37596]

Immunohistochemistry-Paraffin: Otx2 Antibody (1H12G8B2) - BSA Free [NBP2-37596]

Immunohistochemistry-Paraffin: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis of colon tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.
Western Blot: Otx2 Antibody (1H12G8B2)BSA Free [NBP2-37596]

Western Blot: Otx2 Antibody (1H12G8B2)BSA Free [NBP2-37596]

Western Blot: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis using OTX2 mAb against human OTX2 (AA: 40-297) recombinant protein. (Expected MW is 65 kDa)
Immunohistochemistry-Paraffin: Otx2 Antibody (1H12G8B2) - BSA Free [NBP2-37596]

Immunohistochemistry-Paraffin: Otx2 Antibody (1H12G8B2) - BSA Free [NBP2-37596]

Immunohistochemistry-Paraffin: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis of stomach tissues (left) and brain tissues (right) using OTX2 mouse mAb with DAB staining.
ELISA: Otx2 Antibody (1H12G8B2) - BSA Free [NBP2-37596]

ELISA: Otx2 Antibody (1H12G8B2) - BSA Free [NBP2-37596]

ELISA: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Otx2 Antibody (1H12G8B2)

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Otx2

Homeobox protein Otx2 is a member of the bicoid sub-family of homeodomain-containing transcription factors. It is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sense organs. Two distinct isoforms have been identified. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Long Name

Orthodenticle Homolog 2

Alternate Names

homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2

Gene Symbol

OTX2

Additional Otx2 Products

Product Documents for Otx2 Antibody (1H12G8B2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Otx2 Antibody (1H12G8B2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Otx2 Antibody (1H12G8B2)

There are currently no reviews for this product. Be the first to review Otx2 Antibody (1H12G8B2) and earn rewards!

Have you used Otx2 Antibody (1H12G8B2)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies