p27/Kip1 Antibody (4B4-E6) - Azide and BSA Free

Novus Biologicals | Catalog # H00001027-M01

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4B4-E6

Format

Azide and BSA Free
View all p27/Kip1 Primary Antibodies »

Product Specifications

Immunogen

CDKN1B (AAH01971, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDGSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATGDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT

Specificity

CDKN1B - cyclin-dependent kinase inhibitor 1B (p27, Kip1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for p27/Kip1 Antibody (4B4-E6) - Azide and BSA Free

Western Blot: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Western Blot: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Western Blot: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Analysis of CDKN1B expression in transfected 293T cell line by CDKN1B monoclonal antibody (M01), clone 4B4-E6.Lane 1: CDKN1B transfected lysate(22.1 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Analysis of monoclonal antibody to CDKN1B on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Analysis of monoclonal antibody to CDKN1B on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. Antibody concentration 5 ug/ml.
Western Blot: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Western Blot: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Western Blot: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Western Blot analysis of CDKN1B expression in HL-60 ( Cat # L014V1 ).
Proximity Ligation Assay: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Proximity Ligation Assay: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Proximity Ligation Assay: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Analysis of protein-protein interactions between AKT1 and CDKN1B. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-CDKN1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Detection limit for recombinant GST tagged CDKN1B is 10 ng/ml as a capture antibody.
Sandwich ELISA: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Detection limit for recombinant GST tagged CDKN1B is 0.03 ng/ml as a capture antibody.

Applications for p27/Kip1 Antibody (4B4-E6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p27/Kip1

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Entrez Gene IDs

1027 (Human)

Gene Symbol

CDKN1B

OMIM

600778 (Human)

UniProt

AAH01971 (Human)

Additional p27/Kip1 Products

Product Documents for p27/Kip1 Antibody (4B4-E6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for p27/Kip1 Antibody (4B4-E6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Cancer Biomarkers
Cell Cycle

Citations for p27/Kip1 Antibody (4B4-E6) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail