p27/Kip1 Antibody (SX53G8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-34718

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SX53G8

Format

Azide and BSA Free
View all p27/Kip1 Primary Antibodies »

Product Specifications

Immunogen

Purified GST-p27 fusion protein of human origin (Uniprot: P46527)

Localization

Nuclear

Specificity

This monoclonal antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32889).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for p27/Kip1 Antibody (SX53G8) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718]

Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718]

Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (SX53G8) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - p27/Kip1 antibody in CODEX stain of human bone marrow tissue. Image from verified customer review.
Flow Cytometry: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718]

Flow Cytometry: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718]

Flow Cytometry: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Flow Cytometry of human p27/Kip1 on HeLa cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled p27/Kip1 Monoclonal Antibody (SX53G8).
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Formalin-paraffin human colon stained with p27 MAb (SX53G8)
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Formalin-fixed, paraffin-embedded human prostate Carcinoma stained with p27 Monoclonal Antibody (sx53G8)
Flow Cytometry: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718]

Flow Cytometry: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718]

Flow Cytometry: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] - Analysis of PFA-fixed MCF-7 cells using p27/Kip1 Antibody (SX53G8) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).
p27/Kip1 Antibody (SX53G8) - Azide and BSA Free Western Blot: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] -

Western Blot: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] -

Western blot analysis of Raw lysate using p27/Kip1 Antibody (SX53G8) - Azide and BSA Free.
p27/Kip1 Antibody (SX53G8) - Azide and BSA Free Western Blot: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] -

Western Blot: p27/Kip1 Antibody (SX53G8) - Azide and BSA Free [NBP2-34718] -

Western blot analysis of 3T3 lysate using p27/Kip1 Antibody (SX53G8) - Azide and BSA Free.

Applications for p27/Kip1 Antibody (SX53G8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-34718 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: p27/Kip1

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Gene Symbol

CDKN1B

Additional p27/Kip1 Products

Product Documents for p27/Kip1 Antibody (SX53G8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for p27/Kip1 Antibody (SX53G8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Cancer Biomarkers
Cell Cycle

Customer Reviews for p27/Kip1 Antibody (SX53G8) - Azide and BSA Free (1)

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Customer Images

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  • p27/Kip1 Antibody (SX53G8)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: bone marrow
    Species: Human
    Verified Customer | Posted 10/25/2022
    p27 antibody in CODEX stain of human bone marrow tissue
    p27/Kip1 Antibody (SX53G8) - Azide and BSA Free NBP2-34718

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Protocols

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FAQs

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail