P2Y14/GPR105 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93909

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all P2Y14/GPR105 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 209-338 of human P2RY14 (NP_055694.3). ITKKIFKSHLKSSRNSTSVKKKSSRNIFSIVFVFFVCFVPYHIARIPYTKSQTEAHYSCQSKEILRYMKEFTLLLSAANVCLDPIIYFFLCQPFREILCKKLHIPLKAQNDLDISRIKRGNTTLESTDTL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for P2Y14/GPR105 Antibody - BSA Free

Immunohistochemistry-Paraffin: P2Y14/GPR105 Antibody - BSA Free [NBP2-93909]

Immunohistochemistry-Paraffin: P2Y14/GPR105 Antibody - BSA Free [NBP2-93909]

Immunohistochemistry-Paraffin: P2Y14/GPR105 Antibody [NBP2-93909] - Immunohistochemistry of paraffin-embedded rat ovary using P2Y14/GPR105 Rabbit pAb (NBP2-93909) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
P2Y14/GPR105 Antibody

Western Blot: P2Y14/GPR105 Antibody [NBP2-93909] -

Western Blot: P2Y14/GPR105 Antibody [NBP2-93909] - analysis of K-562, using P2RY14 Rabbit pAb at 1:1500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.

Applications for P2Y14/GPR105 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: P2Y14/GPR105

GPR105 is a Sugar-Sensing Receptor. GPR105 is a G-protein-coupled receptor identifying a quiescent, primitive population of hematopoietic cells restricted to bone marrow. GPC105 has been suggested to mediates primitive cell responses to specific hematopoietic microenvironments and extends the known immune system functions of P2Y receptors to the stem cell level. GPR105 has also been suggested to play an important role in peripheral and neuroimmune function in response to extracellular UDP-sugars. GPR105 has been reported to be expressed in brain. ESTs have been isolated from kidney, placenta, uterus, and heart/melanocyte/uterus libraries.

Long Name

P2Y Purinergic Receptor 14

Alternate Names

GPR105, P2RY14

Gene Symbol

P2RY14

Additional P2Y14/GPR105 Products

Product Documents for P2Y14/GPR105 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for P2Y14/GPR105 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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