P4HA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84397

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all P4HA1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDTYNLDTDTISKGNLPGVKHKSFLTAEDC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for P4HA1 Antibody - BSA Free

Western Blot: P4HA1 Antibody [NBP1-84397]

Western Blot: P4HA1 Antibody [NBP1-84397]

Western Blot: P4HA1 Antibody [NBP1-84397] - Analysis using Anti-P4HA1 antibody NBP1-84397 (A) shows similar pattern to independent antibody NBP1-84398 (B).
Immunocytochemistry/ Immunofluorescence: P4HA1 Antibody [NBP1-84397]

Immunocytochemistry/ Immunofluorescence: P4HA1 Antibody [NBP1-84397]

Immunocytochemistry/Immunofluorescence: P4HA1 Antibody [NBP1-84397] - Staining of human cell line U-251 MG shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397] - Staining of human skin shows weak granular cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397] - Staining of human endometrium shows moderate granular cytoplasmic positivity in in glandular cells.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397] - Staining of human kidney shows weak granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.

Applications for P4HA1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P4HA1

P4HA1 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Long Name

Prolyl 4-Hydroxylase Subunit Alpha 1

Alternate Names

4-PH Alpha-1, P4HA

Gene Symbol

P4HA1

Additional P4HA1 Products

Product Documents for P4HA1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for P4HA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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