P4HA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84398

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, EA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all P4HA1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DYYHTELWMEQALRQLDEGEISTIDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for P4HA1 Antibody - BSA Free

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining in human placenta and pancreas tissues using NBP1-84398 antibody. Corresponding P4HA1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: P4HA1 Antibody [NBP1-84398]

Immunocytochemistry/ Immunofluorescence: P4HA1 Antibody [NBP1-84398]

Immunocytochemistry/Immunofluorescence: P4HA1 Antibody [NBP1-84398] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human cerebellum shows moderate granular cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human placenta shows strong cytoplasmic positivity in decidual cells.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.
P4HA1 Antibody - BSA Free Western Blot: P4HA1 Antibody - BSA Free [NBP1-84398]

Western Blot: P4HA1 Antibody - BSA Free [NBP1-84398]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
P4HA1 Antibody - BSA Free Western Blot: P4HA1 Antibody - BSA Free [NBP1-84398]

Western Blot: P4HA1 Antibody - BSA Free [NBP1-84398]

Analysis in human cell lines PC-3 and HEK293 using Anti-P4HA1 antibody. Corresponding P4HA1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Applications for P4HA1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-84398 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P4HA1

P4HA1 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Long Name

Prolyl 4-Hydroxylase Subunit Alpha 1

Alternate Names

4-PH Alpha-1, P4HA

Gene Symbol

P4HA1

Additional P4HA1 Products

Product Documents for P4HA1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for P4HA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for P4HA1 Antibody - BSA Free

Customer Reviews for P4HA1 Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
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1 Stars
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  • P4HA1 Antibody
    Name: Pinyuan Tian
    Application: Western Blot
    Sample Tested: Immortalized human podocytes
    Species: Human
    Verified Customer | Posted 10/24/2022
    western blot of P4HA1 in human podocyte
    P4HA1 Antibody - BSA Free NBP1-84398
  • P4HA1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: U87MG whole cell lysate
    Species: Human
    Verified Customer | Posted 04/18/2017
    Antibody used at 1:1000 dilution
    P4HA1 Antibody - BSA Free NBP1-84398

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Protocols

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FAQs

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