p53 [p Thr55] Antibody (3C3) - BSA Free

Novus Biologicals | Catalog # NBP3-26386

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3C3 expressed in HEK293

Format

BSA Free
View all p53 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human TP53 [p Thr55] [UniProt P04637]

Modification

p Thr55

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p53 [p Thr55] Antibody (3C3) - BSA Free

p53 [p Thr55] Antibody (3C3)

Western Blot: p53 [p Thr55] Antibody (3C3) [NBP3-26386] -

Western Blot: p53 [p Thr55] Antibody (3C3) [NBP3-26386] - Positive Western Blot detected in 293 whole cell lysate (treated with Calyculin A or Pervanadate).
All lanes: p53 [p Thr55] Antibody at 1.28ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 53 KDa
Observed band size: 53 KDa
p53 [p Thr55] Antibody (3C3)

Immunocytochemistry/Immunofluorescence: p53 [p Thr55] Antibody (3C3) [NBP3-26386] -

Immunocytochemistry/Immunofluorescence: p53 [p Thr55] Antibody (3C3) [NBP3-26386] - Staining of 293 cells (treated with 50mM Calyculin A for 30min) with p53 [p Thr55] Antibody (3C3) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for p53 [p Thr55] Antibody (3C3) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

Additional p53 Products

Product Documents for p53 [p Thr55] Antibody (3C3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for p53 [p Thr55] Antibody (3C3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for p53 [p Thr55] Antibody (3C3) - BSA Free

There are currently no reviews for this product. Be the first to review p53 [p Thr55] Antibody (3C3) - BSA Free and earn rewards!

Have you used p53 [p Thr55] Antibody (3C3) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies