p53 Antibody (Pab DO-1) [Alexa Fluor® 647]

Novus Biologicals | Catalog # NBP2-50538AF647

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # Pab DO-1
View all p53 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human wild type p53 protein expressed in E.coli

Specificity

DO-1 recognises wild-type and mutant p53. DO-1 recognises three of the p53 isoforms (p53, p53 beta, p53 gamma).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for p53 Antibody (Pab DO-1) [Alexa Fluor® 647]

p53 Antibody (Pab DO-1) [Alexa Fluor® 647]

Flow Cytometry: Mouse p53 mAb (Pab DO-1) [Alexa Fluor® 647] [NBP2-50538AF647]

Flow Cytometry: Mouse p53 mAb (Pab DO-1) [Alexa Fluor® 647] [NBP2-50538AF647] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review.

Applications for p53 Antibody (Pab DO-1) [Alexa Fluor® 647]

Application
Recommended Usage

Flow Cytometry

Validated for Flow Cytometry from a verified customer review.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-50538AF647 in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

Additional p53 Products

Product Documents for p53 Antibody (Pab DO-1) [Alexa Fluor® 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for p53 Antibody (Pab DO-1) [Alexa Fluor® 647]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for p53 Antibody (Pab DO-1) [Alexa Fluor® 647] (1)

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Customer Images

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  • p53 Antibody (Pab DO-1) [Alexa Fluor® 647]
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 04/02/2025
    P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment
    p53 Antibody (Pab DO-1) [Alexa Fluor® 647] NBP2-50538AF647
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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