p53 [p Ser33] Antibody (SR1942) - BSA Free

Novus Biologicals | Catalog # NBP3-21514

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1942 expressed in HEK293

Format

BSA Free
View all p53 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-p53 [Ser33] (Uniprot #: P04637)

Modification

p Ser 33

Specificity

Detects endogenous levels of total phospho-p53 [Ser33]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p53 [p Ser33] Antibody (SR1942) - BSA Free

Western Blot: p53 [p Ser 33] Antibody (SR1942) [NBP3-21514] -

Western Blot: p53 [p Ser 33] Antibody (SR1942) [NBP3-21514] - Western blot analysis using NBP3-21514 in T47D cell lysates treated with etoposide.

Applications for p53 [p Ser33] Antibody (SR1942) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

Additional p53 Products

Product Documents for p53 [p Ser33] Antibody (SR1942) - BSA Free

Certificate of Analysis

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Product Specific Notices for p53 [p Ser33] Antibody (SR1942) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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