p97/VCP Antibody (5H12) - BSA Free

Novus Biologicals | Catalog # NBP3-26257

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5H12 expressed in HEK293

Format

BSA Free
View all VCP Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human p97/VCP [UniProt P55072]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p97/VCP Antibody (5H12) - BSA Free

p97/VCP Antibody (5H12)

Immunoprecipitation: p97/VCP Antibody (5H12) [NBP3-26257] -

Immunoprecipitation: p97/VCP Antibody (5H12) [NBP3-26257] - Immunoprecipitating p97/VCP in U251 whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26257 in U251 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26257 (2ug) + U251 whole cell lysate (500ug).
Lane 3: U251 whole cell lysate (10ug).
p97/VCP Antibody (5H12)

Immunohistochemistry: p97/VCP Antibody (5H12) [NBP3-26257] -

Immunohistochemistry: p97/VCP Antibody (5H12) [NBP3-26257] - Image of p97/VCP Antibody (5H12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
p97/VCP Antibody (5H12)

Immunohistochemistry: p97/VCP Antibody (5H12) [NBP3-26257] -

Immunohistochemistry: p97/VCP Antibody (5H12) [NBP3-26257] - Image of p97/VCP Antibody (5H12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
p97/VCP Antibody (5H12)

Western Blot: p97/VCP Antibody (5H12) [NBP3-26257] -

Western Blot: p97/VCP Antibody (5H12) [NBP3-26257] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, Rat brain tissue, Rat liver tissue.
All lanes: p97/VCP Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 90 kDa
Observed band size: 90 kDa
p97/VCP Antibody (5H12)

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (5H12) [NBP3-26257] -

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (5H12) [NBP3-26257] - Staining of SY5Y Cells with p97/VCP Antibody (5H12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for p97/VCP Antibody (5H12) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: VCP

Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease.

Long Name

Valosin-containing Protein

Alternate Names

IBMPFD, TERA

Gene Symbol

VCP

Additional VCP Products

Product Documents for p97/VCP Antibody (5H12) - BSA Free

Certificate of Analysis

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Product Specific Notices for p97/VCP Antibody (5H12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
NFkB Pathway

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Protocols

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