p97/VCP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81620

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VCP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p97/VCP Antibody - BSA Free

Western Blot: p97/VCP Antibody [NBP1-81620]

Western Blot: p97/VCP Antibody [NBP1-81620]

Western Blot: p97/VCP Antibody [NBP1-81620] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VCP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody [NBP1-81620]

Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody [NBP1-81620]

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody [NBP1-81620] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP1-81620]

Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP1-81620]

Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP1-81620] - Staining of human hippocampus shows strong nuclear and cytoplasmic positivity in neuronal cells and glial cells.
Western Blot: p97/VCP Antibody [NBP1-81620]

Western Blot: p97/VCP Antibody [NBP1-81620]

Western Blot: p97/VCP Antibody [NBP1-81620] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for p97/VCP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VCP

Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease.

Long Name

Valosin-containing Protein

Alternate Names

IBMPFD, TERA

Gene Symbol

VCP

Additional VCP Products

Product Documents for p97/VCP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for p97/VCP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
NFkB Pathway

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Protocols

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FAQs

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