Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rabbit (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PAK2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human PAK2. The immunogen is located within amino acids 20 - 70 of PAK2. Amino Acid Squence: PLPSVPEEKKPRHK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit PAK2 Antibody - BSA Free (NBP1-76721) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PAK2 Antibody - BSA Free

PAK2 Antibody - BSA Free

Western Blot: PAK2 Antibody - BSA Free [NBP1-76721] -

Western Blot: PAK2 Antibody - BSA Free [NBP1-76721] - Validation in Mouse TissuesLoading: 15 ug of lysates per lane.Antibodies: PAK2 (1 ug/mL per lane), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
PAK2 Antibody - BSA Free

Immunohistochemistry: PAK2 Antibody - BSA Free [NBP1-76721] -

Immunohistochemistry: PAK2 Antibody - BSA Free [NBP1-76721] - Validation of PAK2 in Mouse Spleen Tissue. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PAK2 antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
PAK2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PAK2 Antibody - BSA Free [NBP1-76721] -

Immunocytochemistry/ Immunofluorescence: PAK2 Antibody - BSA Free [NBP1-76721] - Validation of PAK2 in Mouse Spleen TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen tissue labeling PAK2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
PAK2 Antibody - BSA Free

Western Blot: PAK2 Antibody - BSA Free [NBP1-76721] -

Western Blot: PAK2 Antibody - BSA Free [NBP1-76721] - Validation in Jurkat LysateLoading: 15 ug of lysates per lane.Antibodies: PAK2 (A: 0.5 ug/mL, B: 1 ug/mL and C: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: PAK2 AntibodyBSA Free [NBP1-76721]

Western Blot: PAK2 AntibodyBSA Free [NBP1-76721]

Western Blot: PAK2 Antibody [NBP1-76721] - Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: PAK2 NBP1-76720 (1 ug/mL), PAK2 NBP1-76721 (1 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
PAK2 Antibody - BSA Free

Western Blot: PAK2 Antibody - BSA Free [NBP1-76721] -

Western Blot: PAK2 Antibody - BSA Free [NBP1-76721] - Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: PAK2 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Applications for PAK2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAK2

The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.

Long Name

p21-activated Kinase 2

Alternate Names

gamma-PAK, PAK65, S6/H4 Kinase

Gene Symbol

PAK2

UniProt

NP_002568 (Human)

Additional PAK2 Products

Product Documents for PAK2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PAK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Intracellular Kinases

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Protocols

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