Palladin Antibody (1E6) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80902

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat, Canine, Chicken

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1E6

Format

Azide and BSA Free
View all Palladin Primary Antibodies »

Product Specifications

Immunogen

Endogenous Palladin immunoprecipitated from chicken embryo fibroblasts.

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 23451256).

Localization

Cytoplasm, cytoskeleton. Note: Localizes to stress fibers and Z-disks. Cell junction, focal adhesion. Cell projection, ruffle. Cell projection, lamellipodium.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Palladin Antibody (1E6) - Azide and BSA Free

Western Blot: Palladin Antibody (1E6)Azide and BSA Free [NBP2-80902]

Western Blot: Palladin Antibody (1E6)Azide and BSA Free [NBP2-80902]

Western Blot: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902] - Western blot analysis of HeLa (A), A431 (B), NIH3T3 (C), and PC-12 (D) cell line extracts using Palladin antibody at 1ug/ml. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902]

Immunocytochemistry/ Immunofluorescence: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902]

Immunocytochemistry/Immunofluorescence: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902] - Immunofluorescence on a7r5 cells. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902]

Immunohistochemistry-Paraffin: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902]

Immunohistochemistry-Paraffin: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902] - Palladin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Palladin (1E6) Monoclonal Antibody (Catalog # NBP1-25959) at 1:100 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisU
Flow Cytometry: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902]

Flow Cytometry: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902]

Flow Cytometry: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902] - An intracellular stain was performed on HeLa cells with Palladin Antibody [1E6] NBP1-25959AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.
Western Blot: Palladin Antibody (1E6)Azide and BSA Free [NBP2-80902]

Western Blot: Palladin Antibody (1E6)Azide and BSA Free [NBP2-80902]

Western Blot: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902] - MDA-MB-231 lysate. Image from the standard format of this antibody.
Western Blot: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902]

Western Blot: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902]

Western Blot: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902] - Western blots analysis of C2C12 cells labeled with a monoclonal antibody against 90-kDa palladin or a polyclonal antibody against 140- and 200-kDa palladin, MyHC, myogenin, and MLC. The blots clearly show the decrease in palladin expression concomitant with the elevation of differentiation markers. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0124762) licensed under a CC-BY license. Image from the standard format of this antibody.
Knockdown Validated: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902]

Immunocytochemistry/ Immunofluorescence: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902]

Immunocytochemistry/ Immunofluorescence: Palladin Antibody (1E6) - Azide and BSA Free [NBP2-80902] - C2C12 cells were transfected with shRNA targeting palladin or nontargeting shRNA as a control. Stable transfectants were submitted to a differentiation medium and followed for 5 days in culture (D0 to D5). Myogenic differentiation was assessed at the indicated time points using RNA/protein analysis and immunofluorescence staining. Immunofluorescence images of stable transfectants labeled with palladin antibody (green) and F-actin (red). Blue is DAPI-stained nuclei. Scale bar is 10 um. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0124762) licensed under a CC-BY license. Image from the standard format of this antibody.

Applications for Palladin Antibody (1E6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

5 ug/ml

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:100

Immunoprecipitation

0.05 ml / 2 ml lysate

Western Blot

1 ug/ml
Application Notes
In WB a band is seen at ~85 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Palladin

Palladin (PALLD) is an actin associated cytoskeletal protein which is widely expressed and found in stress fibers, focal adhesions, growth cones, Z-discs, and other actin-based subcellular structures. It belongs to a small gene family that includes the Z-disc proteins myopalladin and myotilin, all of which share similar Ig-like domains and interact with specific molecular partners: all three bind to alpha-actinin; in addition, palladin also binds to VASP and ezrin, myotilin binds to filamin and actin, and myopalladin also binds to nebulin and CARP. While myotilin and myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. Palladin functions as a molecular scaffold as well as an actin cross linker, and its nuclear presence and ability to translocate to the nucleus upon stimulation reflects that palladin has signaling functions in excitation-transcription. It interacts with EPS8, LASP1, VASP, ACTN, ARGBP2, LPP, PFN1, SPIN90, SRC and EZR, and plays key role in smooth muscle differentiation, migration, contraction, embryonic development and in some pathological conditions including cardiovascular diseases as well as cancers. Palladin levels are typically upregulated actively migrating cells e.g. developing vertebrate embryos, in cells along a wound edge, and in metastatic cancer cells; and genetic variations in PALLD gene have been linked with susceptibility to pancreatic cancer type 1 (PNCA1) and myocardial infarction.

Alternate Names

PALLD, PNCA1, SIH002

Gene Symbol

PALLD

Additional Palladin Products

Product Documents for Palladin Antibody (1E6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Palladin Antibody (1E6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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