Pannexin-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92241

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Pannexin-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VLKVYEILPTFDVLHFKSEGYNDLSLYNLFLEENISEVKSYKCLKVLENIKSSGQGIDPMLLLTNLGMIKMDVVDGKTPMSAEMREEQGNQTAELQGMNIDSETKANNGEKNARQRLLDSSC

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pannexin-1 Antibody - BSA Free

Western Blot: Pannexin-1 Antibody [NBP1-92241]

Western Blot: Pannexin-1 Antibody [NBP1-92241]

Western Blot: Pannexin-1 Antibody [NBP1-92241] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PANX1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Pannexin-1 Antibody [NBP1-92241]

Immunocytochemistry/ Immunofluorescence: Pannexin-1 Antibody [NBP1-92241]

Immunocytochemistry/Immunofluorescence: Pannexin-1 Antibody [NBP1-92241] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241]

Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241]

Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human small intestine shows moderate membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241]

Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241]

Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241]

Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241]

Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241]

Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241]

Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human pancreas shows negative to very weak positivity in exocrine glandular cells.
Pannexin-1 Antibody - BSA Free Western Blot: Pannexin-1 Antibody - BSA Free [NBP1-92241]

Western Blot: Pannexin-1 Antibody - BSA Free [NBP1-92241]

Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Applications for Pannexin-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pannexin-1

The protein encoded by the Pannexin 1 gene belongs to the innexin family. Innexin family members are the structural components ofgap junctions. This protein and pannexin 2 are abundantly expressed in central nerve system (CNS) and are coexpressedin various neuronal populations. Studies in Xenopus oocytes suggest that this protein alone and in combination withpannexin 2 may form cell type-specific gap junctions with distinct properties. (provided by RefSeq)

Alternate Names

Innexin, MRS1, Pannexin1, PANX1, PX1

Gene Symbol

PANX1

Additional Pannexin-1 Products

Product Documents for Pannexin-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Pannexin-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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