PAR/pADPr Antibody

Catalog #: 4336-APC-050
8 Citations Datasheet / COA / SDS
Newer Version Available: 4335-MC-100

Discontinued Product

4336-APC-050 has been discontinued and is replaced by 4335-MC-100.

Detection of PAR/pADPr by Western Blot.
1 Image
Product Details
Citations (8)

PAR/pADPr Antibody Summary

This polyclonal antibody detects free PAR and poly-ribosylated proteins.
Polyclonal Rabbit IgG
Antigen Affinity-purified
Poly(ADP-ribose) polymer
This antibody is an affinity purified IgG fraction in 1X PBS, containing 50% glycerol.


Recommended Concentration
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of PAR/pADPr antibody by Western Blot. View Larger

Detection of PAR/pADPr by Western Blot. Western blot analysis of Wehi cells untreated (H) and treated (T) with 25 μM Etoposide for 4 hours at 37 °C. Cells were lysed in Tris-Glycine SDS sample buffer at the concentration of 1 x 107cells/ml, and 10 μl of clarified lysate were loaded per well of 4-20% Tris-Glycine gel. Proteins were transferred onto an Immobilon FL membrane and ribosylated proteins were detected using Trevigen's polyclonal anti-PAR antibody (cat# 4336-APC-050) followed by an IR680-conjugated secondary antibody (Licor). The membrane was scanned using an Odyssey Infrared Imaging System (Licor).

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
-20 °C (manual defrost freezer)

Background: PAR/pADPr

Procedure for Immunoblotting using Peroxidase Detection:

Blotting buffer: 12 mM Tris base, 96 mM Glycine, and 15% MeOH.
Blocking solution: 5% (w/v) nonfat dry milk in TBS-0.1% Tween.
Antibody solution: 5% (w/v) nonfat dry milk, in TBS-0.1% Tween.

Transfer the electrophoresed proteins to a PVDF membrane and incubate the membrane for 1 hour at room temperature in blocking solution.

Incubate the membrane overnight at 4°C in antibody solution containing a 1:1000 dilution of anti-PAR rabbit polyclonal antibody. Empirical determination of primary antibody concentration will be required for optimal results.

Wash the membrane at room temperature for 5 minutes with 3 changes of TBS-0.1% Tween. Changing the membrane containers often reduces background.

Incubate the membrane at room temperature for 1 hour in antibody solution containing antirabbit conjugated to horseradish peroxidase.

Empirical determination of secondary antibody concentration will be required for optimal results.

Wash the membrane for 5 minutes with 4 changes of TBS-0.1% Tween.

Develop peroxidase reaction using chemiluminescence.

  1. Affar, E.B., et al. 1998. Immunodot blot method for the detection of poly(ADP-ribose) synthesized in vitro and in vivo. Anal Biochem 259:280-3.
  2. Shah, G.M., et al. 1995. Methods for biochemical study of poly(ADP-ribose) metabolism in vitro and in vivo. Anal Biochem 227:1-13.
  3. Gagné, J-P., et al. 2008. Proteome-wide identification of poly(ADP-ribose) binding proteins and poly(ADP-ribose)-associated protein complexes. Nucleic Acids Res 36:6959-76.
Long Name
Poly [ADP-ribose] Polymer
Alternate Names
pADPr; PAR; Poly(ADP-ribose)

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Citations for PAR/pADPr Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Deletion of Topoisomerase 1 in excitatory neurons causes genomic instability and early onset neurodegeneration
    Authors: G Fragola, AM Mabb, B Taylor-Bla, JK Niehaus, WD Chronister, H Mao, JM Simon, H Yuan, Z Li, MJ McConnell, MJ Zylka
    Nat Commun, 2020-04-23;11(1):1962.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. The nuclear structural protein NuMA is a negative regulator of 53BP1 in DNA double-strand break repair
    Authors: N Salvador M, J Liu, KM Haas, LL Parker, C Chakrabort, SJ Kron, K Hodges, LD Miller, C Langefeld, PJ Robinson, SA Lelièvre, PA Vidi
    Nucleic Acids Res., 2019-04-08;0(0):.
    Species: Human
    Sample Types: Nuclear Extract
    Applications: Western Blot
  3. An uncharacterized FMAG_01619 protein from Fusobacterium mortiferum ATCC 9817 demonstrates that some bacterial macrodomains can also act as poly-ADP-ribosylhydrolases
    Authors: AG García-Sau, R Zapata-Pér, JF Hidalgo, J Cabanes, F Gil-Ortiz, Á Sánchez-Fe
    Sci Rep, 2019-03-01;9(1):3230.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  4. NR4A Nuclear Receptors Target Poly-ADP-Ribosylated DNA-PKcs Protein to Promote DNA Repair
    Authors: D Munnur, J Somers, G Skalka, R Weston, R Jukes-Jone, M Bhogadia, C Dominguez, K Cain, I Ahel, M Malewicz
    Cell Rep, 2019-02-19;26(8):2028-2036.e6.
    Species: Human
    Sample Types: Cell Extracts
    Applications: Western Blot
  5. RANKL coordinates multiple osteoclastogenic pathways by regulating expression of ubiquitin ligase RNF146.
    Authors: Matsumoto Y, Larose J, Kent O, Lim M, Changoor A, Zhang L, Storozhuk Y, Mao X, Grynpas M, Cong F, Rottapel R
    J Clin Invest, 2017-03-13;127(4):1303-1315.
  6. Cultured networks of excitatory projection neurons and inhibitory interneurons for studying human cortical neurotoxicity.
    Authors: Xu J, Fan J, Wang X, Eacker S, Kam T, Chen L, Yin X, Zhu J, Chi Z, Jiang H, Chen R, Dawson T, Dawson V
    Sci Transl Med, 2016-04-06;8(333):333ra48.
  7. The 3'-5' DNA exonuclease TREX1 directly interacts with poly(ADP-ribose) polymerase-1 (PARP1) during the DNA damage response.
    Authors: Miyazaki T, Kim Y, Yoon J, Wang H, Suzuki T, Morse H
    J Biol Chem, 2014-10-02;289(47):32548-58.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. NuMA promotes homologous recombination repair by regulating the accumulation of the ISWI ATPase SNF2h at DNA breaks.
    Authors: Vidi P, Liu J, Salles D, Jayaraman S, Dorfman G, Gray M, Abad P, Moghe P, Irudayaraj J, Wiesmuller L, Lelievre S
    Nucleic Acids Res, 2014-04-20;42(10):6365-79.


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