Patched 1/PTCH Antibody - BSA Free
Novus Biologicals | Catalog # NB200-118
Key Product Details
Species Reactivity
Validated:
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Applications
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Label
Antibody Source
Format
Product Specifications
Immunogen
Reactivity Notes
Specificity
Clonality
Host
Isotype
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Patched 1/PTCH Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody - BSA Free [NB200-118]](https://resources.rndsystems.com/images/products/Patched-1-PTCH-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB200-118-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody - BSA Free [NB200-118]")
Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody - BSA Free [NB200-118]
Patched-1-PTCH-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB200-118-img0003.jpg![Immunohistochemistry: Patched 1/PTCH Antibody - BSA Free [NB200-118]](https://resources.rndsystems.com/images/products/Patched-1-PTCH-Antibody---BSA-Free-Immunohistochemistry-NB200-118-img0002.jpg "Immunohistochemistry: Patched 1/PTCH Antibody - BSA Free [NB200-118]")
Immunohistochemistry: Patched 1/PTCH Antibody - BSA Free [NB200-118]
Patched-1-PTCH-Antibody---BSA-Free-Immunohistochemistry-NB200-118-img0002.jpg![Western Blot: Patched 1/PTCH AntibodyBSA Free [NB200-118]](https://resources.rndsystems.com/images/products/Patched-1-PTCH-Antibody---BSA-Free-Western-Blot-NB200-118-img0001.jpg "Western Blot: Patched 1/PTCH AntibodyBSA Free [NB200-118]")
Western Blot: Patched 1/PTCH AntibodyBSA Free [NB200-118]
Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Analysis of Human PTCH1 in Fetal brain Lysate with anti huPTCH1 at a 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody - BSA Free [NB200-118] -
Increased Ihh mRNA and protein levels and activity in Smad2 and Smad3 mutant growth plates.All images are P0 proximal tibias (A) RNA in situ hybridization for Ihh showing increased expression in Smad2CKO, Smad3-/-, and double mutant growth plates. (B) Immunostaining for Ihh protein showing diffusion into the resting zones of Smad2CKO, Smad3-/-, and double mutant growth plates. (C) Magnifications of the boxed regions in (B). (D) Immunostaining for Ptch1, showing increased protein levels throughout the growth plate in mutants, particularly in the resting zone. (E) Immunostaining for phospho-Smad1/5/8 (PS158) protein showing no significant difference in levels of activated BMP signaling comparing Smad2CKO, Smad3-/-, or double mutant to control Smad2fx/fx growth plates. Smad2CKO = Smad2fx/fx;Col2a1Cre. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27741240), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Patched 1/PTCH Antibody - BSA Free
ELISA
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Frozen
Immunohistochemistry-Paraffin
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Format
Preservative
Concentration
Shipping
Stability & Storage
Background: Patched 1/PTCH
Alternate Names
Gene Symbol
Additional Patched 1/PTCH Products
Product Documents for Patched 1/PTCH Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Patched 1/PTCH Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Patched 1/PTCH Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Patched 1/PTCH Antibody - BSA Free
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Q: Could you please tell me an alternative for NBP1-52497?
A: An appropriate replacement would be NB200-118.
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Q: Does this antibody detect an epitope within the first extracellular loop of Ptch1? Is the residue 269-279 within this loop?
A:
According to the UniProt entry (http://www.uniprot.org/uniprot/Q13635), the first extracellular loop corresponds to amino acids 122-436, so yes, the immunogen/epitope is within this region.
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Q: I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?
A:
NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.
-
Q: Could you please tell me an alternative for NBP1-52497?
A: An appropriate replacement would be NB200-118.
-
Q: Does this antibody detect an epitope within the first extracellular loop of Ptch1? Is the residue 269-279 within this loop?
A:
According to the UniProt entry (http://www.uniprot.org/uniprot/Q13635), the first extracellular loop corresponds to amino acids 122-436, so yes, the immunogen/epitope is within this region.
-
Q: I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?
A:
NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.
-
Q: Could you please tell me an alternative for NBP1-52497?
A: An appropriate replacement would be NB200-118.
-
Q: Does this antibody detect an epitope within the first extracellular loop of Ptch1? Is the residue 269-279 within this loop?
A:
According to the UniProt entry (http://www.uniprot.org/uniprot/Q13635), the first extracellular loop corresponds to amino acids 122-436, so yes, the immunogen/epitope is within this region.
-
Q: I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?
A:
NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.