Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Pax2 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Pax2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Pax2 Antibody - Azide and BSA Free

Western Blot: Pax2 Antibody [NBP2-99013]

Western Blot: Pax2 Antibody [NBP2-99013]

Western Blot: Pax2 Antibody [NBP2-99013] - Lane A: K562 Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:44 kDa
Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013]

Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013]

Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013] - Staining of human Pax2 in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution.
Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013]

Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013]

Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013] - Staining of human Pax2 in human kidney with rabbit polyclonal antibody at 1:1000 dilution.
Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013]

Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013]

Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013] - Staining of human Pax2 in human renal carcinoma with rabbit polyclonal antibody at 1:1000.
Immunoprecipitation: Pax2 Antibody [NBP2-99013]

Immunoprecipitation: Pax2 Antibody [NBP2-99013]

Immunoprecipitation: Pax2 Antibody [NBP2-99013] - Pax2 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. 4 ul anti-Pax2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Pax2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 45 kDa
Pax2 Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Pax2 Antibody [NBP2-99013] -

Immunocytochemistry/Immunofluorescence: Pax2 Antibody [NBP2-99013] - Immunofluorescence staining of Pax2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99013 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

Applications for Pax2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

0.5-2uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pax2

PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor supressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants.

Long Name

Paired Box Gene 2

Alternate Names

paired box 2, paired box gene 2, paired box homeotic gene 2, paired box protein Pax-2

Gene Symbol

PAX2

Additional Pax2 Products

Product Documents for Pax2 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Pax2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Pax2 Antibody - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Pax2 Antibody - Azide and BSA Free and earn rewards!

Have you used Pax2 Antibody - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies