Pax6 Antibody (GT9412) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13636

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT9412

Format

Azide and BSA Free
View all Pax6 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pax6. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Pax6 Antibody (GT9412) - Azide and BSA Free

Western Blot: Pax6 Antibody (GT9412) [NBP3-13636]

Western Blot: Pax6 Antibody (GT9412) [NBP3-13636]

Western Blot: Pax6 Antibody (GT9412) [NBP3-13636] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636]

Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636]

Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636] - Pax6 antibody [GT9412] detects Pax6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Pax6 stained by Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Pax6 Antibody (GT9412)

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody (GT9412) [NBP3-13636] -

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody (GT9412) [NBP3-13636] - Pax6 antibody [GT9412] detects Pax6 protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pax6 stained by Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.

Applications for Pax6 Antibody (GT9412) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pax6

PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters® anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.

Long Name

Paired Box Gene 6

Alternate Names

keratitis), MGC17209, Oculorhombin, paired box 6, paired box protein Pax-6

Gene Symbol

PAX6

Additional Pax6 Products

Product Documents for Pax6 Antibody (GT9412) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Pax6 Antibody (GT9412) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for Pax6 Antibody (GT9412) - Azide and BSA Free

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  • Q: What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?

    A:

    By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.

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