Paxillin [p Tyr118] Antibody (GT712) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13552

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chicken (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT712

Format

Azide and BSA Free
View all Paxillin Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr118 of human Paxillin. The exact sequence is proprietary.

Modification

p Tyr 118

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Paxillin [p Tyr118] Antibody (GT712) - Azide and BSA Free

Western Blot: Paxillin [p Tyr118] Antibody (GT712) [NBP3-13552] - Wild-type (WT) and Paxillin (phospho Tyr118) knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (NBP3-13552) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/Immunofluorescence: Paxillin [p Tyr118] Antibody (GT712) [NBP3-13552] - Paxillin (phospho Tyr118) antibody [GT712] detects Paxillin (phospho Tyr118) protein at focal adhesion by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Paxillin (phospho Tyr118) stained by Paxillin (phospho Tyr118) antibody [GT712] (NBP3-13552) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Fluoroshield with DAPI. Scale bar= 10 um.
Immunohistochemistry-Paraffin: Paxillin [p Tyr118] Antibody (GT712) [NBP3-13552] - Paxillin (phospho Tyr118) antibody [GT712] detects Paxillin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Paxillin stained by Paxillin (phospho Tyr118) antibody [GT712] (NBP3-13552) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Paxillin [p Tyr118] Antibody (GT712) [NBP3-13552] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (NBP3-13552) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: Paxillin [p Tyr118] Antibody (GT712) [NBP3-13552] - Untreated (-) and treated (+) A431 whole cell extracts (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (NBP3-13552) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Applications for Paxillin [p Tyr118] Antibody (GT712) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Paxillin

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

Alternate Names

PXN

Gene Symbol

PXN

Additional Paxillin Products

Product Documents for Paxillin [p Tyr118] Antibody (GT712) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Paxillin [p Tyr118] Antibody (GT712) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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