PBK Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84342

Novus Biologicals
Loading...

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
Loading...

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSNVVIKGDFETIKICDVGVSLPLDENMTVTDPEACYIGTEPWKPKEAVEENGVITDKADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDFDDEAYYAALGTRPPINMEELDESYQKVIELFSVCTNEDPKDRP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PBK Antibody - BSA Free

Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342]

Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342]

Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human colon, kidney, liver and testis using Anti-PBK antibody NBP1-84342 (A) shows similar protein distribution across tissues to independent antibody NBP2-58043 (B).
Immunocytochemistry/ Immunofluorescence: PBK Antibody [NBP1-84342]

Immunocytochemistry/ Immunofluorescence: PBK Antibody [NBP1-84342]

Immunocytochemistry/Immunofluorescence: PBK Antibody [NBP1-84342] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342]

Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342]

Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human testis.
Immunohistochemistry: PBK Antibody [NBP1-84342]

Immunohistochemistry: PBK Antibody [NBP1-84342]

Immunohistochemistry: PBK Antibody [NBP1-84342] - Staining of mouse brain shows positivity in rostral migratory stream.
Immunohistochemistry: PBK Antibody [NBP1-84342]

Immunohistochemistry: PBK Antibody [NBP1-84342]

Immunohistochemistry: PBK Antibody [NBP1-84342] - Staining of mouse medulla shows positivity in a subset of cells.
Immunohistochemistry: PBK Antibody [NBP1-84342]

Immunohistochemistry: PBK Antibody [NBP1-84342]

Immunohistochemistry: PBK Antibody [NBP1-84342] - Staining of mouse subventricular zone shows immunoreactivity in some cells in the rostral migratory stream.
Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342]

Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342]

Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human colon.
Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342]

Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342]

Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human kidney.
Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342]

Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342]

Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human liver.
PBK Antibody - BSA Free Western Blot: PBK Antibody - BSA Free [NBP1-84342]

Western Blot: PBK Antibody - BSA Free [NBP1-84342]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
PBK Antibody - BSA Free Immunohistochemistry: PBK Antibody - BSA Free [NBP1-84342]

Immunohistochemistry: PBK Antibody - BSA Free [NBP1-84342]

Staining of mouse olfactory bulb shows immunoreactivity in a subset of neurons.
PBK Antibody - BSA Free Western Blot: PBK Antibody - BSA Free [NBP1-84342]

Western Blot: PBK Antibody - BSA Free [NBP1-84342]

Analysis in human cell line A-431.

Applications for PBK Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PBK

PBK encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.

Alternate Names

Cancer/testis antigen 84, CT84MAPKK-like protein kinase, EC 2.7.12.2, FLJ14385, Nori-3PDZ-binding kinase, PDZ binding kinase, serine/threonine protein kinase, Spermatogenesis-related protein kinase, SPKT-LAK cell-originated protein kinase, TOPKlymphokine-activated killer T-cell-originated protein kinase

Gene Symbol

PBK

Additional PBK Products

Product Documents for PBK Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PBK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PBK Antibody - BSA Free

Customer Reviews for PBK Antibody - BSA Free

There are currently no reviews for this product. Be the first to review PBK Antibody - BSA Free and earn rewards!

Have you used PBK Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...