PBX1 Antibody (4A2) - Azide and BSA Free

Novus Biologicals | Catalog # H00005087-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Monkey

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4A2

Format

Azide and BSA Free
View all PBX1 Primary Antibodies »

Product Specifications

Immunogen

PBX1 (NP_002576, 213 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQLKQSTCEAVMILRSRFLDARRKRRNFNKQATEILNEYFYSHLSNPYPSEEAKEELAKKCGITVSQVSNWFGNKRIRYKKNIGKFQEEANIYAAKTAVTATNVSAHGS

Specificity

PBX1 - pre-B-cell leukemia transcription factor 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PBX1 Antibody (4A2) - Azide and BSA Free

Western Blot: PBX1 Antibody (4A2) [H00005087-M01]

Western Blot: PBX1 Antibody (4A2) [H00005087-M01]

Western Blot: PBX1 Antibody (4A2) [H00005087-M01] - PBX1 monoclonal antibody (M01), clone 4A2 Analysis of PBX1 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: PBX1 Antibody (4A2) [H00005087-M01]

Immunocytochemistry/ Immunofluorescence: PBX1 Antibody (4A2) [H00005087-M01]

Immunocytochemistry/Immunofluorescence: PBX1 Antibody (4A2) [H00005087-M01] - Analysis of monoclonal antibody to PBX1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: PBX1 Antibody (4A2) [H00005087-M01]

Immunohistochemistry-Paraffin: PBX1 Antibody (4A2) [H00005087-M01]

Immunohistochemistry-Paraffin: PBX1 Antibody (4A2) [H00005087-M01] - Analysis of monoclonal antibody to PBX1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
Sandwich ELISA: PBX1 Antibody (4A2) [H00005087-M01] - Detection limit for recombinant GST tagged PBX1 is approximately 0.03ng/ml as a capture antibody.

Applications for PBX1 Antibody (4A2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 26610607).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PBX1

PBX1 binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development a

Alternate Names

DKFZp686B09108, Homeobox protein PBX1, Homeobox protein PRL, MGC126627, Pre-B cell leukemia transcription factor-1, pre-B-cell leukemia homeobox 1, pre-B-cell leukemia transcription factor 1, PRL

Entrez Gene IDs

5087 (Human)

Gene Symbol

PBX1

OMIM

176310 (Human)

UniProt

NP_002576 (Human)

Additional PBX1 Products

Product Documents for PBX1 Antibody (4A2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PBX1 Antibody (4A2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PBX1 Antibody (4A2) - Azide and BSA Free

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Protocols

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FAQs

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