PBX1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85803

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PBX1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PRLMHSHAGVGMAGHPGLSQHLQDGAGGTEGEGGRKQDIGDILQQIMTITDQSLDEAQARKHALNCHRMKPALFNVLCEIKEKTVLSIRGAQEEEPTDPQLMRLDNMLLAEGV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PBX1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803]

Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803]

Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803] - Analysis in human fallopian tube and liver tissues using NBP1-85803 antibody. Corresponding PBX1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803]

Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803]

Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803] - Staining of human endometrium, fallopian tube, liver and prostate using Anti-PBX1 antibody NBP1-85803 (A) shows similar protein distribution across tissues to independent antibody NBP1-86151 (B).
Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803]

Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803]

Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803] - Staining of human prostate shows weak to moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803]

Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803]

Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803]

Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803]

Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803] - Staining of human endometrium shows moderate to strong nuclear positivity in stromal cells.
Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803]

Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803]

Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803] - Staining of human liver shows no positivity in hepatocytes as expected.
PBX1 Antibody - BSA Free Western Blot: PBX1 Antibody - BSA Free [NBP1-85803]

Western Blot: PBX1 Antibody - BSA Free [NBP1-85803]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
PBX1 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: PBX1 Antibody - BSA Free [NBP1-85803]

Immunocytochemistry/ Immunofluorescence: PBX1 Antibody - BSA Free [NBP1-85803]

Staining of human cell line U-251 MG shows localization to nucleoplasm.
PBX1 Antibody - BSA Free Western Blot: PBX1 Antibody - BSA Free [NBP1-85803]

Western Blot: PBX1 Antibody - BSA Free [NBP1-85803]

Analysis in human cell lines HeLa and SK-MEL-30 using Anti-PBX1 antibody. Corresponding PBX1 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Applications for PBX1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PBX1

PBX1 binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development a

Alternate Names

DKFZp686B09108, Homeobox protein PBX1, Homeobox protein PRL, MGC126627, Pre-B cell leukemia transcription factor-1, pre-B-cell leukemia homeobox 1, pre-B-cell leukemia transcription factor 1, PRL

Gene Symbol

PBX1

Additional PBX1 Products

Product Documents for PBX1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PBX1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PBX1 Antibody - BSA Free

Customer Reviews for PBX1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review PBX1 Antibody - BSA Free and earn rewards!

Have you used PBX1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies