Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4C2

Format

BSA Free
View all PDCD4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human PDCD4 (1-469aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for PDCD4 Antibody (4C2) - BSA Free

Western Blot: PDCD4 Antibody (4C2) [NBP3-18224]

Western Blot: PDCD4 Antibody (4C2) [NBP3-18224]

Western Blot: PDCD4 Antibody (4C2) [NBP3-18224] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP3-18224 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: K562 cell lysate. Lane 2: HeLa cell lysate. Lane 3: 293T cell lysate
Immunocytochemistry/ Immunofluorescence: PDCD4 Antibody (4C2) [NBP3-18224]

Immunocytochemistry/ Immunofluorescence: PDCD4 Antibody (4C2) [NBP3-18224]

Immunocytochemistry/Immunofluorescence: PDCD4 Antibody (4C2) [NBP3-18224] - ICC/IF analysis of PDCD4 in HeLa cells. The cell was stained with NBP3-18224 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry: PDCD4 Antibody (4C2) [NBP3-18224]

Flow Cytometry: PDCD4 Antibody (4C2) [NBP3-18224]

Flow Cytometry: PDCD4 Antibody (4C2) [NBP3-18224] - Flow cytometry analysis of PDCD4 in MCF7 cells. The cell was stained with NBP3-18224 at 2-5ug for 1x10^6cells (red). A Goat anti-mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Applications for PDCD4 Antibody (4C2) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate-Buffered Saline (pH 7.4), 10% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDCD4

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1 responsive promoters by c-Jun.

Long Name

Programmed Cell Death 4

Alternate Names

H731, Protein 197/15a

Gene Symbol

PDCD4

Additional PDCD4 Products

Product Documents for PDCD4 Antibody (4C2) - BSA Free

Certificate of Analysis

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Product Specific Notices for PDCD4 Antibody (4C2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Translation Regulation

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Protocols

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FAQs

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