PDCD4 [p Ser457] Antibody

Novus Biologicals | Catalog # NBP3-13143

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Chicken (100%), Human (100%), Rhesus Macaque (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PDCD4 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser457 of human PDCD4. The exact sequence is proprietary.

Modification

p Ser 457

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for PDCD4 [p Ser457] Antibody

Western Blot: PDCD4 [p Ser457] Antibody [NBP3-13143]

Western Blot: PDCD4 [p Ser457] Antibody [NBP3-13143]

Western Blot: PDCD4 [p Ser457] Antibody [NBP3-13143] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PDCD4 (phospho Ser457) antibody (NBP3-13143) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PDCD4 [p Ser457] Antibody [NBP3-13143]

Immunohistochemistry-Paraffin: PDCD4 [p Ser457] Antibody [NBP3-13143]

Immunohistochemistry-Paraffin: PDCD4 [p Ser457] Antibody [NBP3-13143] - PDCD4 (phospho Ser457) antibody detects PDCD4 (phospho Ser457) protein at nucleus in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. PDCD4 (phospho Ser457) antibody (NBP3-13143) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: PDCD4 [p Ser457] Antibody [NBP3-13143]

Western Blot: PDCD4 [p Ser457] Antibody [NBP3-13143]

Western Blot: PDCD4 [p Ser457] Antibody [NBP3-13143] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PDCD4 (phospho Ser457) antibody (NBP3-13143) diluted at 1:1000.
Immunohistochemistry-Paraffin: PDCD4 [p Ser457] Antibody [NBP3-13143]

Immunohistochemistry-Paraffin: PDCD4 [p Ser457] Antibody [NBP3-13143]

Immunohistochemistry-Paraffin: PDCD4 [p Ser457] Antibody [NBP3-13143] - PDCD4 (phospho Ser457) antibody detects PDCD4 (phospho Ser457) protein at nucleus in mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. PDCD4 (phospho Ser457) antibody (NBP3-13143) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for PDCD4 [p Ser457] Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDCD4

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1 responsive promoters by c-Jun.

Long Name

Programmed Cell Death 4

Alternate Names

H731, Protein 197/15a

Gene Symbol

PDCD4

Additional PDCD4 Products

Product Documents for PDCD4 [p Ser457] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PDCD4 [p Ser457] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Translation Regulation

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Protocols

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FAQs

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