PDE10A Antibody

Novus Biologicals | Catalog # NB300-645

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human PDE10A2 protein common to all PDE10A variants.

Localization

Located mostly to soluble cellular fractions.

Specificity

This has no cross-reactivity against any other PDE family members tested so far.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDE10A Antibody

Western Blot: PDE10A Antibody [NB300-645]

Western Blot: PDE10A Antibody [NB300-645]

Western Blot: PDE10A Antibody [NB300-645] - Rat Striatum: 1. Pan-PDE10A antibody. 2. PDE10A2 variant selective antibody. Diluted 1:5000in DiluOBuffer.
Immunohistochemistry-Paraffin: PDE10A Antibody [NB300-645]

Immunohistochemistry-Paraffin: PDE10A Antibody [NB300-645]

Immunohistochemistry-Paraffin: PDE10A Antibody - 1:1000 dilution in IHC Block Buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X mangnification.FFPE section. Mouse Brain.
Western Blot: PDE10A Antibody [NB300-645]

Western Blot: PDE10A Antibody [NB300-645]

Western Blot: PDE10A Antibody [NB300-645] - NB300-645 (1:500) using CNS Blot. 1. Frontal Cortex 2. Pariental Cortex 3. Striatum 4. Amygdela 5. Hypothalamus 6. Hippocampus 7. Olfactory bulb 8. Brain Stem 9. Cerebellum 10. Spinal cord and 11. Whole Brain.
Immunohistochemistry-Paraffin: PDE10A Antibody [NB300-645]

Immunohistochemistry-Paraffin: PDE10A Antibody [NB300-645]

Immunohistochemistry-Paraffin: PDE10A Antibody [NB300-645] - Slide A is negative control. Slide B is stained with NB300-645 at 1:200 antibody dilution in DiluObuffer.

Applications for PDE10A Antibody

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

> 1:250

Western Blot

> 1:500
Application Notes
ICC/IF reactivity reported in (PMID: 21559457). Detects a band of approximately 90-93 kDa in rat brain and rat pancreas samples. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

0.7 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDE10A

One of the many phosphodiesterases that compartmentalize and hydrolyze cAMP and cGMP into AMP and GMP is PDE10. Only one member of the cAMP and cGMP-specific PDE10 family has been cloned and characterized. The PDE10A gene is expressed in at least two variant forms (PDE10A1, PDE10A2) generated by RNA splicing and/or the use of alternate initiation sites. PDE10A is widely expressed in the putamen and caudate nucleus in the brain, thyroid and testis. The expression of PDE10A is also abundant in Heart.

Long Name

cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A

Alternate Names

EC 3.1.4.17

Entrez Gene IDs

10846 (Human); 23984 (Mouse); 63885 (Rat)

Gene Symbol

PDE10A

UniProt

Q9Y233 (Human)

Additional PDE10A Products

Product Documents for PDE10A Antibody

Certificate of Analysis

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Product Specific Notices for PDE10A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PDE10A Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PDE10A Antibody

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  • Q: This website claims that Tian et al (2011) successfully used this Pde10A antibody. However, in this paper they say the antibody that they used is from Scottish Biomedical. Is the antibody that you are selling here the same as they used, just now available from Novus rather than Scottish Biomedical?

    A: I pulled up that article and it in fact does cite Novus as the antibody source. If you read into the Immunocytochemistry section, or do a search on the article for the word Novus it will come up. I believe the Scottish Biomedical reference is for the BSA.

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