![Western Blot: PDE3B Antibody [NBP3-12242]](https://resources.rndsystems.com/images/products/PDE3B-Antibody-Western-Blot-NBP3-12242-img0003.jpg "Western Blot: PDE3B Antibody [NBP3-12242]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Chicken, Primate
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
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Product Specifications
Immunogen
Synthetic peptide taken within amino acid region 1050-2000 on mouse PDE3B protein. Will label all variants of PDE3B.
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for PDE3B Antibody
Western Blot: PDE3B Antibody [NBP3-12242]
Western Blot: PDE3B Antibody [NBP3-12242] - Western Blot![Immunocytochemistry/ Immunofluorescence: PDE3B Antibody [NBP3-12242]](https://resources.rndsystems.com/images/products/PDE3B-Antibody-Immunohistochemistry-Paraffin-NBP3-12242-img0004.jpg "Immunocytochemistry/ Immunofluorescence: PDE3B Antibody [NBP3-12242]")
Immunocytochemistry/ Immunofluorescence: PDE3B Antibody [NBP3-12242]
Immunocytochemistry/Immunofluorescence: PDE3B Antibody [NBP3-12242] - NBP3-12242 on COS1 cells expressing NBP3-12242. 1:100 antibody dilution in Dilobuffer.![Immunohistochemistry-Paraffin: PDE3B Antibody [NBP3-12242]](https://resources.rndsystems.com/images/products/PDE3B-Antibody-Immunohistochemistry-Paraffin-NBP3-12242-img0001.jpg "Immunohistochemistry-Paraffin: PDE3B Antibody [NBP3-12242]")
Immunohistochemistry-Paraffin: PDE3B Antibody [NBP3-12242]
Immunohistochemistry-Paraffin: PDE3B Antibody [NBP3-12242] - IHC antibody with human ovarian medulla tissue. 1:100 antibody dilution.![Western Blot: PDE3B Antibody [NBP3-12242]](https://resources.rndsystems.com/images/products/PDE3B-Antibody-Western-Blot-NBP3-12242-img0002.jpg "Western Blot: PDE3B Antibody [NBP3-12242]")
Western Blot: PDE3B Antibody [NBP3-12242]
Western Blot: PDE3B Antibody [NBP3-12242] - WB of NBP3-12242. 1:500 antibody gilution in DiluObuffer. Apparent MW is 145 kDa.Applications for PDE3B Antibody
Application
Recommended Usage
ELISA
1:10000
Immunocytochemistry/ Immunofluorescence
1:250
Immunohistochemistry
1:250
Immunoprecipitation
1:200
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: PDE3B
Long Name
cGMP-inhibited 3',5'-cyclic phosphodiesterase B
Alternate Names
CGI-PDE B, CGIP1, CGIPDE1
Gene Symbol
PDE3B
Additional PDE3B Products
Product Documents for PDE3B Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PDE3B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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