PDE5A Antibody

Novus Biologicals | Catalog # NBP3-12207

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Sheep

Applications

Immunohistochemistry, Western Blot, ELISA, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
View all PDE5A Primary Antibodies »

Product Specifications

Immunogen

Phosphorylated synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for PDE5A Antibody

Western Blot: PDE5A Antibody [NBP3-12207]

Western Blot: PDE5A Antibody [NBP3-12207]

Western Blot: PDE5A Antibody [NBP3-12207] - WB recombinant NBP3-12207 antibody. Overexpressing cells were treated with or without ANP. 1:500 in antibody dilution in DilObuffer. Blot was stripped with StripObuffer.

Applications for PDE5A Antibody

Application
Recommended Usage

Dot Blot

1:10000

ELISA

1:10000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: PDE5A

PDE5A encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms.

Long Name

cGMP-specific 3',5'-cyclic phosphodiesterase

Alternate Names

CGB-PDE, PDE5

Gene Symbol

PDE5A

Additional PDE5A Products

Product Documents for PDE5A Antibody

Certificate of Analysis

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Product Specific Notices for PDE5A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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