PDGF R beta Antibody (42G12) - BSA Free

Novus Biologicals | Catalog # NBP1-19191

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Proximity Ligation Assay, Knockdown Validated

Cited:

Proximity Ligation Assay, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 42G12

Format

BSA Free
View all PDGF R beta Primary Antibodies »

Product Specifications

Immunogen

Recombinant human PDGF Receptor beta Protein

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

PDGF Receptor beta (42G12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDGF R beta Antibody (42G12) - BSA Free

Western Blot: PDGF R beta Antibody (42G12) [NBP1-19191]

Western Blot: PDGF R beta Antibody (42G12) [NBP1-19191]

Western Blot: PDGF R beta Antibody (42G12) [NBP1-19191] - Molecular Weight Markers (1) and Mouse Heart Muscle lysate (2) probed with PDGF R beta Antibody Monoclonal Antibody (extra bands are secondary reactivity)
Immunohistochemistry-Paraffin: PDGF R beta Antibody (42G12) [NBP1-19191]

Immunohistochemistry-Paraffin: PDGF R beta Antibody (42G12) [NBP1-19191]

Immunohistochemistry-Paraffin: PDGF R beta Antibody (42G12) [NBP1-19191] - Human spleen tissue at 20 ug/ml
Immunohistochemistry-Paraffin: PDGF R beta Antibody (42G12) [NBP1-19191]

Immunohistochemistry-Paraffin: PDGF R beta Antibody (42G12) [NBP1-19191]

Immunohistochemistry-Paraffin: PDGF R beta Antibody (42G12) [NBP1-19191] - Human placenta at 20 ug/ml

Applications for PDGF R beta Antibody (42G12) - BSA Free

Application
Recommended Usage

Immunohistochemistry

20 ug/ml

Immunohistochemistry-Paraffin

20 ug/ml

Western Blot

1:100-1:2000
Application Notes
PLA and knockdown validation (PMID: 31672904).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDGF R beta

Mitogenic platelet derived growth factor (PDGF) signaling is mediated by the PDGF tyrosine kinase receptors PDGFRalpha and PDGFRbeta, which exist as homo- or heterodimer isoforms (alphaalpha, betabeta, alphabeta). Secretion of PDGFs and expression of PDGFRs in a broad distribution of cell types contributes to regulation of cellular processes including cell growth, actin reorganization, prevention of apoptosis, and chemotaxis. Structurally, the transmembrane PDGFRs are composed of an N-terminal extracellular domain with five immunoglobulin repeats, and an intracellular domain containing two spaced tyrosine kinase domains. PDGF ligands display preferential affinity to PDGFRalpha and PDGFRbeta, with PDGFRbeta activation restricted to binding by PDGF BB and PDGF DD. Growth factor binding initiates receptor dimerization and activates the cytosolic tyrosine kinase domains.

Long Name

Platelet-derived Growth Factor Receptor beta

Alternate Names

CD140b, PDGFRB

Entrez Gene IDs

5159 (Human); 18596 (Mouse); 24629 (Rat)

Gene Symbol

PDGFRB

Additional PDGF R beta Products

Product Documents for PDGF R beta Antibody (42G12) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PDGF R beta Antibody (42G12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PDGF R beta Antibody (42G12) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PDGF R beta Antibody (42G12) - BSA Free

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  • Q: What is the buffer formulation and concentration of sodium azide?

    A: It has the formulation of: "Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol".

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