PDGFR alpha Antibody (8E12F2) - BSA Free

Novus Biologicals | Catalog # NBP2-52528

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Feline

Applications

Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8E12F2

Format

BSA Free
View all PDGF R alpha Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PDGFR alpha (AA: 361-528) expressed in E. Coli.

Reactivity Notes

Feline reactivity reported from a verified customer review.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

122.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDGFR alpha Antibody (8E12F2) - BSA Free

Western Blot: PDGFR alpha Antibody (8E12F2)BSA Free [NBP2-52528]

Western Blot: PDGFR alpha Antibody (8E12F2)BSA Free [NBP2-52528]

Western Blot: PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Analysis using PDGFRA mAb against HEK293 (1) and PDGFRA (AA: 361-528)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (8E12F2) - BSA Free [NBP2-52528]

Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (8E12F2) - BSA Free [NBP2-52528]

Immunocytochemistry/Immunofluorescence: PDGFR alpha Antibody (8E12F2) [NBP2-52528] - PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Imaging of Optice Nerve. Primary Antibody (red, dilution1:800) and DAPI (blue). This image was submitted via customer Review.
Flow (Intracellular): PDGFR alpha Antibody (8E12F2) - BSA Free [NBP2-52528]

Flow (Intracellular): PDGFR alpha Antibody (8E12F2) - BSA Free [NBP2-52528]

Flow (Intracellular): PDGF R alpha Antibody (8E12F2) [NBP2-52528] - An intracellular stain was performed on HeLa cells with PDGF R alpha Antibody (8E12F2) NBP2-52528AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Western Blot: PDGFR alpha Antibody (8E12F2)BSA Free [NBP2-52528]

Western Blot: PDGFR alpha Antibody (8E12F2)BSA Free [NBP2-52528]

Western Blot: PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Analysis using PDGFRA mAb against human PDGFRA (AA: 361-528) recombinant protein. (Expected MW is 44.8 kDa)
Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (8E12F2) - BSA Free [NBP2-52528]

Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (8E12F2) - BSA Free [NBP2-52528]

Immunocytochemistry/Immunofluorescence: PDGFR alpha Antibody (8E12F2) [NBP2-52528] - PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Analysis of Hela cells using PDGFRA 8E12F2 HELA 100 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry: PDGFR alpha Antibody (8E12F2) - BSA Free [NBP2-52528]

Flow Cytometry: PDGFR alpha Antibody (8E12F2) - BSA Free [NBP2-52528]

Flow Cytometry: PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Analysis of Hela cells using PDGFRA mouse mAb (green) and negative control (red).
ELISA: PDGFR alpha Antibody (8E12F2) - BSA Free [NBP2-52528]

ELISA: PDGFR alpha Antibody (8E12F2) - BSA Free [NBP2-52528]

ELISA: PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Applications for PDGFR alpha Antibody (8E12F2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Reviewed Applications

Read 2 reviews rated 3 using NBP2-52528 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDGF R alpha

Platelet-derived growth factor receptors exhibit tyrosine-protein kinase activity and have been implicated in the control of cell proliferation, survival and migration. PDGF receptors, PDGFR-alpha and PDGFR-beta, have 5 extracellular immunoglobulin-like domains and an intracellular tyrosine kinase domain. Upon binding a PDGF, the receptors form homo-and heterodimers. Dimerization of the receptors results in phosphorylation in the complex. More than 10 different SH2-domain-containing molecules have been shown to bind to different autophosphorylation sites in the PDGF-alpha and beta receptors. PDGF alpha receptors are expressed in oligodendrocyte progenitor cells and PDGF beta receptors are expressed on neurons.

Long Name

Platelet-derived Growth Factor Receptor alpha

Alternate Names

CD140a, PDGFR alpha, PDGFRA

Gene Symbol

PDGFRA

Additional PDGF R alpha Products

Product Documents for PDGFR alpha Antibody (8E12F2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PDGFR alpha Antibody (8E12F2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PDGFR alpha Antibody (8E12F2) - BSA Free

Customer Reviews for PDGFR alpha Antibody (8E12F2) - BSA Free (2)

3 out of 5
2 Customer Ratings
5 Stars
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3 Stars
100%
2 Stars
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • PDGF R alpha Antibody (8E12F2)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Optic nerve
    Species: Feline
    Verified Customer | Posted 08/08/2017
    NBP2-52528(red, dilution1:800) and DAPI (blue)
    PDGFR alpha Antibody (8E12F2) - BSA Free NBP2-52528
  • PDGF R alpha Antibody (8E12F2)
    Name: Kazuya Oikawa
    Application: Immunocytochemistry
    Sample Tested: Optic nerve
    Species: Feline
    Verified Customer | Posted 05/28/2017
    NBP2-52528(red, dilution1:800) and DAPI (blue)
    PDGFR alpha Antibody (8E12F2) - BSA Free NBP2-52528

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