PDGFR alpha Antibody (APA5) - BSA Free

Novus Biologicals | Catalog # NBP1-43350

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # APA5

Format

BSA Free
View all PDGF R alpha Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD140a.

Reactivity Notes

Human reactivity reported from a verified customer review.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for PDGFR alpha Antibody (APA5) - BSA Free

Flow Cytometry: PDGFR alpha Antibody (APA5) - BSA Free [NBP1-43350]

Flow Cytometry: PDGFR alpha Antibody (APA5) - BSA Free [NBP1-43350]

Flow Cytometry: PDGF R alpha Antibody (APA5) [NBP1-43350] - Analysis using the Biotin conjugate of NBP1-43350. Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140a (PDGF Receptor a) Biotin followed by Streptavidin PE. Appropriate isotype controls were used (open histogram).
Flow Cytometry: PDGFR alpha Antibody (APA5) - BSA Free [NBP1-43350]

Flow Cytometry: PDGFR alpha Antibody (APA5) - BSA Free [NBP1-43350]

Flow Cytometry: PDGF R alpha Antibody (APA5) [NBP1-43350] - Staining of NIH/3T3 cell line with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse CD140a (PDGF Receptor a) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis

Applications for PDGFR alpha Antibody (APA5) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
The APA5 antibody has been tested by flow cytometric analysis of the NIH-3T3 cell line. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8cells/test.

In flow cytometry, we recommend using a cell surface staining protocol and fixation and permeabilization is not required to get staining. If fixation/permeabilization is needed for other markers in the same experiment, we recommend doing all surface staining prior to the fix/perm procedure. This antibody may not bind to a fixed epitope.

Reviewed Applications

Read 1 review rated 5 using NBP1-43350 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PDGF R alpha

The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.

Long Name

Platelet-derived Growth Factor Receptor alpha

Alternate Names

CD140a, PDGFR alpha, PDGFRA

Entrez Gene IDs

18595 (Mouse)

Gene Symbol

PDGFRA

UniProt

P26618

Additional PDGF R alpha Products

Product Documents for PDGFR alpha Antibody (APA5) - BSA Free

Certificate of Analysis

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Product Specific Notices for PDGFR alpha Antibody (APA5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PDGFR alpha Antibody (APA5) - BSA Free (1)

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  • PDGFR alpha Antibody (APA5)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: A549 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 03/07/2020
    30ug protein was loaded each lane and antibody dilution was 1:1000.
    PDGFR alpha Antibody (APA5) - BSA Free NBP1-43350

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